Content Determination of 5 Compositions of Genkwa Flos before and after Processed

LI Lin; GUAN Hong-yue; YIN Fang-zhou; LU Tu-lin; LIN Jia-hong; CAI Bao-chang

doi:10.11653/syfj2013240066

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Content Determination of 5 Compositions of Genkwa Flos before and after Processed

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- Content Determination of 5 Compositions of Genkwa Flos before and after Processed
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 24, Pages: 66-70(2013)
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- DOI：10.11653/syfj2013240066
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- Published：2013
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LI Lin, GUAN Hong-yue, YIN Fang-zhou, et al. Content Determination of 5 Compositions of Genkwa Flos before and after Processed[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 66-70.
DOI：

LI Lin, GUAN Hong-yue, YIN Fang-zhou, et al. Content Determination of 5 Compositions of Genkwa Flos before and after Processed[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 66-70. DOI： 10.11653/syfj2013240066.

摘要

目的：建立HPLC-MS同时测定芫花中木犀草素、芹菜素、羟基芫花素、芫花素以及芫花酯甲5种化学成分含量的方法，分析炮制前后含量的变化。方法：采用液质联用法，质谱采用大气压电喷雾离子源，选择性负离子模式监测。色谱柱为Agilent Zorbax C18（2.1 mm×50 mm，1.8 μm），流动相为0.05%甲酸乙腈（A）-0.05%甲酸水（B），流速0.6 mL·min-1，柱温30℃，进样量2 μL。结果：芫花炮制后5种毒效成分均发生了一定程度的变化。3种黄酮类有效成分木犀草素、羟基芫花素以及芫花素炮制后含量升高，二萜原酸酯甲代表成分芫花酯甲炮制后含量降低，黄酮类成分芹菜素经炮制后含量也发生了下降。结论：该方法简单、灵敏、准确，为芫花及其炮制品的质量评价提供了有效的方法。

Abstract

Objective: To establish a HPLC-MS method for the simultaneous quantitation of luteolin

apigenin

3'-hydroxy-genkwanin

genkwanin and yuanhuacine in Genkwa Flos

and to analyze the content change before and after processing. Method: HPLC-MS was used for quantification in negative ion mode under ESI. Chromatography was performed on Agilent Zorbax C18(2.1 mm×50 mm

1.8 μm) column by gradient elution using 0.05% formic acid in acetonitrile-0.05% formic acid in water at the flow rate of 0.6 mL·min-1. The column temperature was kept at 30℃ and the inject volume was 2 μL. Result: The content of five toxic and active compounds was all changed after processed. The content of the luteolin

3'-hydroxy-genkwanin

genkwanin was higher and the apigenin and yuanhuacine were lower than the raw ones. Conclusion: The new developed LC-MS method is simple

sensitive and accurate. It can be used for the quality evaluation of crude and processed Genkwa Flos.

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