Determination of Condensed Tannins in Spatholobi Caulis

LIN Hui-zhen; LIU Hao-wen; WEI Wei; ZHOU Ying-li; CHEN Jian-ping; WANG Dong-mei

doi:10.11653/syfj2013240070

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Determination of Condensed Tannins in Spatholobi Caulis

更新时间：2024-01-04

- Determination of Condensed Tannins in Spatholobi Caulis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 24, Pages: 70-74(2013)
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- DOI：10.11653/syfj2013240070
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- Published：2013
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LIN Hui-zhen, LIU Hao-wen, WEI Wei, et al. Determination of Condensed Tannins in Spatholobi Caulis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 70-74.
DOI：

LIN Hui-zhen, LIU Hao-wen, WEI Wei, et al. Determination of Condensed Tannins in Spatholobi Caulis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 70-74. DOI： 10.11653/syfj2013240070.

摘要

目的：建立鸡血藤药材中缩合鞣质的含量测定方法，并对不同批次鸡血藤药材及其不同部位中缩合鞣质的含量进行测定。方法：优化并建立了采用香草醛-硫酸显色体系测定鸡血藤药材中缩合鞣质含量的方法，反应条件为3 mL 3%香草醛，3 mL 40%浓硫酸，常温反应20 min，测定波长为500 nm。结果： 5个批次鸡血藤药材中缩合鞣质含量为3.99%~8.63%；其中3个批次药材不同部位的缩合鞣质含量测定结果显示，树脂状分泌物中含量最高，为8.14%~11.51%，木质部（5.39%~7.92%）和韧皮部（3.65%~6.90%）含量相对较少。结论：香草醛-硫酸显色法快速、简便、准确、重复性好，可用于测定鸡血藤药材中缩合鞣质的含量；缩合鞣质在树脂状分泌物中的含量比其他部位高，为传统的鸡血藤药材质量的判断方法提供了科学依据。

Abstract

Objective: To establish a method for determination of condensed tannins in Spatholobi Caulis (the stem of Suberect spatholobus) and investigate the content of condensed tannins in Spatholobi Caulis and its different parts. Method: The vanillin-sulphuric acid coloration system for the determination of condensed tannins was set up. The optimized condition of coloration was 3 mL 3% vanillin

3 mL 40% sulphuric acid

at room temperature for 20 min

and the detection wavelength was set at 500 nm. Result: The content of condensed tannins was measured as 3.99%-8.63% in five commercial crude drugs of Spatholobi Caulis. Among different parts of the crude drugs

condensed tannins was most abundant in the resin-like secretion part (8.14%-11.51%)

far higher than those in the xylem part (5.39%-7.92%) and the phloem part (3.65%-6.90%). Conclusion: The developed methods are simple

rapid

accurate

stable

and reproducible with high selectivity

and are suitable for the determination of condensed tannins in the quality control of Spatholobi Caulis.

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