Simultaneous Determination of Three Active Components in  by HPLC

ZHANG Jun-ying; FENG Chao; XU Xue; ZHANG Lei; WU Chun-yong

doi:10.11653/syfj2013240078

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Simultaneous Determination of Three Active Components in by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Three Active Components in by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 24, Pages: 78-81(2013)
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- DOI：10.11653/syfj2013240078
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- Published：2013
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ZHANG Jun-ying, FENG Chao, XU Xue, et al. Simultaneous Determination of Three Active Components in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 78-81.
DOI：

ZHANG Jun-ying, FENG Chao, XU Xue, et al. Simultaneous Determination of Three Active Components in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 78-81. DOI： 10.11653/syfj2013240078.

摘要

目的：建立一种同时测定香蜂花药材中迷迭香酸、咖啡酸和咖啡酸乙酯含量的RP-HPLC分析法。方法： Hanbon Megres C18色谱柱（4.6 mm×250 mm， 5 μm），流动相A为甲酸-乙腈-水（0.5:20:80），B为甲酸-甲醇-乙腈（0.5:40:60），梯度洗脱（0%~25 min，0%~45% B；25%~30 min，45%~100% B；30%~30.1 min，100%~0% B），流速1.0 mL·min-1，柱温35℃，检测波长330 nm。结果：迷迭香酸、咖啡酸和咖啡酸乙酯分别在6.0~300.0，0.15~7.5 mg·L-1和0.15~7.5 mg·L-1线性关系良好，平均加样回收率（n=6）分别为99.48%，99.56%，101.7%。结论：该方法准确，简便，适用于香蜂花药材的质量分析检验。

Abstract

Objective: The purpose of this study is to simultaneously determine the content of rosmarinic acid

caffeic acid and ethyl caffeate in Melissa officinalis by RP-HPLC. Method: Hanbon Megres C18 column (4.6 mm×250 mm

5 μm) was adopted. The mobile phase consisted of formic acid-acetonitrile-water (0.5:20:80) and formic acid-methanol-acetonitrile (0.5:40:60) with gradient elution program(0-25 min

0%-45% B;25-30 min

45%-100% B;30-30.1 min

100%-0% B). The flow rate was 1.0 mL·min-1. The column temperature was set at 35℃ and the detection wavelength was 330 nm. Result: The linear ranges of rosmarinic acid

caffeic acid and ethyl caffeate were 6.0-300.0

0.15-7.5 and 0.15-7.5 mg·L-1 respectively. The average recoveries(n=6) for the sample preparation of the markers were 99.48%

99.56%

and 101.7% respectively. Conclusion: The quantitative method for rosmarinic acid

caffeic acid and ethyl caffeate by HPLC can provide the basis for quality control of M. officinalis.

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Related Institution

Shandong University of Traditional Chinese Medicine

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