Objective: To establish a HPLC method for quantification of quercetin and kaempferol in compound Ganyan granules. Method: Samples were extracted in 80% methanol with ultrasonic process and hydrolyzed for 1 h. The HPLC was performed on an ODS-C18column (4.6 mm×250 mm

5 μm) with methanol-0.4% H3PO4 (50:50) as mobile phase. The flow rate was 1.0 mL·min-1 and the column tempreture was kept at 30℃. The eluate was detected at 360 nm. Result: The linear ranges of quercetin and kaempferol were 0.050 1-0.501 μg (r=0.999 9) and 0.024 2-0.242 μg (r=0.999 5) respectively. The average recoveries of quercetin and kaempferol were 99.69%(RSD 0.22%) and 99.30%(RSD 0.82%). Conclusion: The method can be used for quality control of Ganyan granules. It is simple

rapid and accurate for simultancous determination of two constituents in compound Ganyan granules.