Analysis on Fat-soluble Constituents in Flowers of Tibetan Medicinal Plants of Three Species by GC-MS

LIN Yan; HAN He-dong; HU Hai-qing; LI Rong-jiao; LI Yan; WANG Xiao-ling

doi:10.11653/syfj2013240102

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Analysis on Fat-soluble Constituents in Flowers of Tibetan Medicinal Plants of Three Species by GC-MS

更新时间：2024-01-04

- Analysis on Fat-soluble Constituents in Flowers of Tibetan Medicinal Plants of Three Species by GC-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 24, Pages: 102-107(2013)
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- DOI：10.11653/syfj2013240102
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- Published：2013
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LIN Yan, HAN He-dong, HU Hai-qing, et al. Analysis on Fat-soluble Constituents in Flowers of Tibetan Medicinal Plants of Three Species by GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 102-107.
DOI：

LIN Yan, HAN He-dong, HU Hai-qing, et al. Analysis on Fat-soluble Constituents in Flowers of Tibetan Medicinal Plants of Three Species by GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 102-107. DOI： 10.11653/syfj2013240102.

摘要

目的：分析西藏秦艽花，麻花秦艽花和川西秦艽花脂溶性的成分。方法：采用索氏提取法获得脂溶性成分，经GC-MS技术结合计算机检索对其化学成分进行分离和鉴定，用色谱峰面积归一化法计算各个组分峰的相对含量。结果：从西藏秦艽花，麻花秦艽花和川西秦艽花的脂溶性成分中分别鉴定了38，35，27个化合物，占其各自总量的94.31%，83.09%，80.84%。3种秦艽花脂溶性成分存在一定程度的差异，西藏秦艽花的主要成分为饱和烷烃（59.33%）、醇类（14.53%）、有机酸（6.43%）、酮类（3.84%），麻花秦艽花的主要成分为饱和烷烃（28.24%）、醇类（17.51%）、有机酸（8.09%）、酯类（7.55%），川西秦艽花的主要成分为饱和烷烃（24.79%）、醇类（5.79%）、生物碱（17.03%）、醌类（16.60%）、酯类（2.78%）。其中川西秦艽花的脂溶性成分跟其他2种的区别较大，预示其药理活性的不同。结论：通过对其脂溶性成分和含量的分析评价与比较，为开发利用秦艽花资源提供科学依据。

Abstract

Objective: To analyze fat-soluble constituents in flowers of Tibetan medicinal plants of three Gentiana species

G. dendrologi

G. straminea and G. tibetica. Method: The fat-soluble components were extracted by Soxhlet extraction

separated and identified using GC-MS. The relative content of each component was determined by area normalization. Result: Thrity-eight

thrity-five and twenty-seven compounds from G. dendrologi

G. straminea and G. tibetica were identified respectively. The ratios of identification of total compounds were 94.31%

83.09% and 80.84% respectively. There are some differences among three Gentiana

the main ingredients being alkane (59.33%)

ethanol (14.53%)

and organic acid (6.43%) and ketones (3.84%) in flowers of G. dendrologi

alkane (28.24%)

ethanol (17.51%)

and organic acid (8.09%) and esters (7.55%) in G. straminea

and alkane (24.79%)

ethanol (5.79%)

and alkaloids (17.03%)

quinones (16.60%) and esters (2.78%) in G. tibetica G. tibetica showed different pharmacological activity owing to different chemical compositions. Conclusion: By analyzing and evaluating their fat-soluble components in three Gentiana Species

the scientific data can be provided for exploitation of Gentiana resources.

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