GC-MS Analysis and Biological Activity of Essential Oil from Leaves of

LIU Min-jie; ZHAO Qi; LIANG Na; LIU Ming-chuan; YANG Sheng-jie; YANG Song

doi:10.11653/syfj2013240135

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GC-MS Analysis and Biological Activity of Essential Oil from Leaves of

更新时间：2024-01-04

- GC-MS Analysis and Biological Activity of Essential Oil from Leaves of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 24, Pages: 135-138(2013)
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- DOI：10.11653/syfj2013240135
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- Published：2013
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LIU Min-jie, ZHAO Qi, LIANG Na, et al. GC-MS Analysis and Biological Activity of Essential Oil from Leaves of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 135-138.
DOI：

LIU Min-jie, ZHAO Qi, LIANG Na, et al. GC-MS Analysis and Biological Activity of Essential Oil from Leaves of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 135-138. DOI： 10.11653/syfj2013240135.

摘要

目的：研究黔产盾叶唐松草Thalictrum ichangense叶挥发油的化学成分及其抗氧化和抗肿瘤的生物活性。方法：采用超临界CO2萃取法提取盾叶唐松草叶的挥发油，通过气相色谱-质谱联用技术（GC-MS）分析其化学成分，用色谱峰面积归一法计算各组分相对百分含量，并分别采用DPPH·法和MTT法研究其抗氧化和抗肿瘤的生物活性。结果：从超临界CO2萃取法提取得到的挥发油中鉴定了21个化学成分，已鉴定出的组分占挥发油总量的91.86%；在300 mg·L-1时，挥发油对DPPH·清除率为（61.2±2.1）%，对MGC-803胃癌细胞体外增殖抑制率为（56.0±6.8）%。结论：盾叶唐松草叶挥发油中主要化学成分为正四十四烷（Tetratetracontane，25.82%）、3，5-二烯豆甾烷（Stigmastan-3，5-diene，16.39%）、二十八烷（Octacosane，14.96%）等。盾叶唐松草叶挥发油具有较好的抗氧化活性和对MCG-803体外增殖的抑制作用。

Abstract

Objective: To study the chemical compositions of the essential oil from the leaves of Thalictrum ichangense. Method: The essential oil was extracted by supercritical carbon dioxide extraction assay

and the constituents were identified by gas chromatography-mass spectrometer (GC-MS). The DPPH·method was used to study its antioxidant activity and MTT assay was used to study its anti-tumor activity. The relative content of each component was calculated by area normalization method. Result: Twenty one compounds were identified

representing 91.86% of the essential oil. The scavenging ratio for the DPPH·radicals reaches (61.2±2.1)% when the concentration was 300 mg·L-1 and the essential oil at 300 mg·L-1 showed anti-tumor activities against MGC-803 in vitro with a inhibition rate of (56.0±6.8)%. Conclusion: The principal chemical constituents of the essential oil were tetratetracontane (25.82%)

stigmastan-3

5-diene (16.39%)

and octacosane (14.96%). The essential oil showed certain antioxidant activity and displayed good inhibition proliferation of MCG-803 in vitro. The analytic results can provide further exploitation and utilization of T. ichangense.

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