Investigation of Solubility of Dehydrosilybin and Its Intestinal Absorption Characteristics in Rats

XU Ying; JIN Xue-feng; TONG Shan-shan; CHEN Mei; XU Xi-ming

doi:10.11653/syfj2013240165

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Investigation of Solubility of Dehydrosilybin and Its Intestinal Absorption Characteristics in Rats

更新时间：2024-01-04

- Investigation of Solubility of Dehydrosilybin and Its Intestinal Absorption Characteristics in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 24, Pages: 165-168(2013)
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- DOI：10.11653/syfj2013240165
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- Published：2013
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XU Ying, JIN Xue-feng, TONG Shan-shan, et al. Investigation of Solubility of Dehydrosilybin and Its Intestinal Absorption Characteristics in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 165-168.
DOI：

XU Ying, JIN Xue-feng, TONG Shan-shan, et al. Investigation of Solubility of Dehydrosilybin and Its Intestinal Absorption Characteristics in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 165-168. DOI： 10.11653/syfj2013240165.

摘要

目的：测定脱氢水飞蓟宾（DHS）的溶解度并探讨DHS在小肠的吸收动力学特征。方法：采用HPLC测定DHS含量，流动相甲醇-0.2%甲酸水溶液（60:40），检测波长255 nm。应用大鼠在体肠循环模型考察DHS在大鼠十二指肠、空肠、回肠的吸收特性。结果： DHS于20，37℃振荡24 h的溶解度分别为0.46，1.23 mg·L-1。DHS在十二指肠、空肠及回肠中单位时间吸收率（n=3， ±s）分别为（17.81±6.37）%，（8.41±2.43）%，（12.95±5.06）%，吸收速率常数（Ka）依次为（0.176±0.069），（0.089±0.053），（0.158±0.058）h-1；DHS在十二指肠的Ka随药物质量浓度的增加基本保持不变。结论：温度对DHS溶解度存在一定影响。DHS在十二指肠、空肠、回肠均有吸收，在十二指肠段吸收量和Ka显著高于空肠段。DHS在小肠的吸收机制以被动扩散为主。

Abstract

Objective: To determine solubility of dehydrosilybin(DHS) and investigate its intestinal absorption kinetics characteristics. Method: HPLC was adopted to determine solubility of DHS with mobile phase of methanol-0.2% formic acid solution(60:40) and detection wavelength of 255 nm.Absorption characteristics of DHS in duodenum

jejunum and ileum was investigated by in situ intestinal circulation model in rats. Result: Solubility of DHS were 0.46

1.23 mg·L-1 oscillated at 20

37℃ for 24 h

respectively.Absorption rate per unit time of DHS in duodenum

jejunum and ileum were (17.81±6.37)%

(8.41±2.43)%

(12.95±5.06)%

absorption rate constant(Ka)of them were (0.176±0.069)

(0.089±0.053)

(0.158±0.058) h-1

respectively;Ka of DHS in duodenum generally kept unchanged with drug concentration increased. Conclusion: Temperature had a certain influence on solubility of DHS.DHS had absorption in duodenum

jejunum and ileum

but absorption amount and Ka in duodenum were significantly higher than them in jejunum.Absorption mechanism of DHS in small intestine was based on passive diffusion.

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