Different Extracts of  Inhibit HO-induced SH-SY5Y Cells Injury

HU Yu; ZHAO Dan; ZHANG Xiao-dan; SUN Dong; HAO Hai-guang; YANG Jing-xian

doi:10.11653/syfj2013240184

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Different Extracts of Inhibit HO-induced SH-SY5Y Cells Injury

更新时间：2024-01-04

- Different Extracts of Inhibit HO-induced SH-SY5Y Cells Injury
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 24, Pages: 184-188(2013)
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- DOI：10.11653/syfj2013240184
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- Published：2013
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HU Yu, ZHAO Dan, ZHANG Xiao-dan, et al. Different Extracts of Inhibit HO-induced SH-SY5Y Cells Injury[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 184-188.
DOI：

HU Yu, ZHAO Dan, ZHANG Xiao-dan, et al. Different Extracts of Inhibit HO-induced SH-SY5Y Cells Injury[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 184-188. DOI： 10.11653/syfj2013240184.

摘要

目的：通过比较独活不同提取物对H2O2 致SH-SY5Y细胞损伤的保护作用，筛选药理活性最强的提取部位方法：以回流提取，溶剂萃取及硅胶柱层析方法得到独活不同提取部位预保护6 h后与250 μmol·L-1 H2O2 共同作用于人神经母细胞瘤细胞株（SH-SY5Y） 24 h，MTT检测细胞活力，并试剂盒法测定细胞内超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量，以及免疫荧光细胞化学法检测脑源性神经营养因子（BDNF）和神经因子-3（NT-3）的表达量结果：独活各提取物除石油醚萃取物外均能救护H2O2 所致SH-SY5Y细胞活力降低，且有明显的量效关系；4种提取物均能增强SOD活性，降低MDA含量，不同提取部位抗氧化作用无差异；石油醚-乙酸乙酯组显著增强BDNF、NT-3蛋白表达（与正常组比较，分别为94.5%和97.5%），其神经营养作用强于其余提取物。结论：独活不同提取部位均可不同程度保护H2O2 损伤SH-SY5Y细胞，其中石油醚-乙酸乙酯组作用最强。

Abstract

Objective: To compare the neuroprotective effect of different extracts of Angelica pubescens on SH-SY5Y cells injury induced by hydrogen peroxide (H2O2) so as to screen the most pharmacologically active extracts. Method: Different extracts were obtained by means of reflux extraction

solvents extraction or silica gel column chromatography. Pro-incubation of different extracts on SH-SY5Y cells for 6 h prior to co-treat with 250 μmol·L-1 H2O2 for 24 h

the cell viability were observed by MTT assay. Meanwhile

the activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) was determined.The expression of brain-derived neurotrophic factor (BDNF) and nerve factor-3 (NT-3) by immunofluorescence was measured. Result: All extracts showed the protective effect against decrease of cell viability induced by H2O2 and there was dose-effect relationship except the extract of petrdeum ether(PE) These extracts enhanced SOD activity and lowered H2O2-induced MDA release. Therefor

they exhibited the similar effect of anti-oxidation;According to increasing the level of BDNF and NT-3(94.5% and 97.5% vs. control

relatively)

we found that the PE-EtOAc extract was better neuroprotective than others. Conclusion: The different extracts of A. pubescens show some protective effect on H2O2 induced SH-SY5Y cells injury and the PE-EtOAc extract has the most pharmacologically activity.

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