Objective: To investigate the impact of Simiao Yongan Tang on the expression of thrombin-treated endothelial cells and neutrophil adhesion and endothelial cell P-select expression. Method: MTT was used to detect endothelial cell viability of serum containing Simiao Yongan Tang 0.2 U·mL-1 thrombin was added to EA.hy926 cells

neutrophils was as a model group

2.5%

5%

10%

20% of the serum containing drugs pretreated endothelial cells 24 h after modeling.Bengal method was used to detect the cell adhesion rate

the flow cytometry and ELISA were used to detect endothelial cell P-selectin expression. Result: 10%

20% serum containing drugs inhibited adhesion rate

10% serum of which was better;the ELISA results showed that 10% serum containing drugs pretreatment could inhibited P-selectin expression;the flow cytometry showed that 10%

20% serum containingdrugs inhibited cell surface P-selectin

10% serum containing drugs was better. Conclusion: Simiao Yongan Tang can inhibit neutrophil adhesion of endothelial cells

this effect may be achieved by inhibiting P-selectin expression in endothelial cells.