Icariin Promotes Metabolism and Inhibites Resorption of Rats Femur Tissue

ZHOU Jian; CHEN Ke-ming; GE Bao-feng; MA Xiao-ni; WANG Jia-qi; GUO Xiao-yu; CHENG Kui; GAO Yu-hai; YAN Li-juan; SHI Wen-gui

doi:10.11653/syfj2013240201

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Icariin Promotes Metabolism and Inhibites Resorption of Rats Femur Tissue

更新时间：2024-01-04

- Icariin Promotes Metabolism and Inhibites Resorption of Rats Femur Tissue
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 24, Pages: 201-205(2013)
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- DOI：10.11653/syfj2013240201
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- Published：2013
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ZHOU Jian, CHEN Ke-ming, GE Bao-feng, et al. Icariin Promotes Metabolism and Inhibites Resorption of Rats Femur Tissue[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 201-205.
DOI：

ZHOU Jian, CHEN Ke-ming, GE Bao-feng, et al. Icariin Promotes Metabolism and Inhibites Resorption of Rats Femur Tissue[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 201-205. DOI： 10.11653/syfj2013240201.

摘要

目的：研究淫羊藿苷对体外培养大鼠股骨组织（骨干和骨骺端）吸收活性的影响。方法：体外分离培养大鼠股骨组织的骨干和骨骺端，48 h后采用终浓度为1×10-5 mol·L-1的淫羊藿苷对体外培养骨干和骨骺端进行处理。测定抗酒石酸酸性磷酸酶（tartrate-resistant acid phosphatase，StrACP）活性；测定培养基中葡萄糖（glucose，Glu）和乳酸（lactic acid，Lac）；Real-Time RT-PCR检测抗酒石酸酸性磷酸酶（tartrate-resistant acid phosphatase，StrACP）、集落刺激因子（macrophage colony stimulating factor，MCSF），组织激酶K（cathepsin K，CTSK）mRNA表达水平。结果： 1×10-5 mol·L-1淫羊藿苷可抑制StrACP活性，增加培养基中乳酸含量和减少培养基中葡萄糖含量，抑制StrACP，MCSF，CTSK mRNA的表达水平。结论：淫羊藿苷抑制体外培养大鼠股骨组织的骨骺端和骨干吸收活性。

Abstract

Objective: To investigate effect of icariin on femoral tissue(diaphyses and metaphysis) resorption and metabolic activity of rats in vitro. Method: The femoral tissue (diaphyses and metaphysis) was isolated which was treated with final concentration of 1×10-5 mol·L-1icariin in culture medium in vitro.The tartrate-resistant acid phosphatase (StrACP) activity

glucose and lactic acid content in culture medium were determined

StrACP

MCSF

CTSK mRNA expression was detected by Real-Time RT-PCR. Result: 1×10-5 mol·L-1 icariin inhibited the activity of StrACP

increased the content of lactic acid and decreased glucose content

and improved StrACP

MCSF

CTSK mRNA expression level. Conclusion: Icariin are adjust femurs tissue metabolic activity and inhibited resorption.

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