Effects of Buyang Huanwu Decoction on Expression of TGF-/Smad3 in Pulmonary Fibrosis Rats

YANG Han; WANG Fei; WANG Quan-lin; ZHONG Zhen-dong; LI Bin; YANG Kun

doi:10.11653/syfj2013240240

您当前的位置：

首页 >

文章列表页 >

Effects of Buyang Huanwu Decoction on Expression of TGF-/Smad3 in Pulmonary Fibrosis Rats

更新时间：2024-01-04

- Effects of Buyang Huanwu Decoction on Expression of TGF-/Smad3 in Pulmonary Fibrosis Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 24, Pages: 240-244(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013240240
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
YANG Han, WANG Fei, WANG Quan-lin, et al. Effects of Buyang Huanwu Decoction on Expression of TGF-/Smad3 in Pulmonary Fibrosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 240-244.
DOI：

YANG Han, WANG Fei, WANG Quan-lin, et al. Effects of Buyang Huanwu Decoction on Expression of TGF-/Smad3 in Pulmonary Fibrosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 240-244. DOI： 10.11653/syfj2013240240.

摘要

目的：观察补阳还五汤对博莱霉素致肺纤维化大鼠肺组织病理及转化生长因子β1（TGF-β1）mRNA，Smad3 mRNA表达的影响。方法：将144只健康雄性SD大鼠随机分成6组，即假手术（NS）组、模型（BLM）组、强的松（P）组、补阳还五汤高、中、低（A，B，C）组，每组各24只。除NS组外，其余各组采用一次性气管滴注博莱霉素复制肺纤维化大鼠模型，NS组气管内滴注等量NS，造模第2天起，A，B，C组用补阳还五汤（剂量分别为18.48，9.24，4.62 g·kg-1）灌胃，P组用强的松混悬液（4.2 mg·kg-1）灌胃，NS组及BLM组用NS（10 mL·kg-1）灌胃，于造模后第7，14，28 d分3批处死动物，每次8只，取左肺制备病理切片，行HE，Masson染色观察肺泡炎及肺纤维化程度改变情况；取右肺上中叶组织，采用逆转录-聚合酶链反应（RT-PCR）法检测各组大鼠肺组织TGF-β1mRNA，Smad3 mRNA的表达。结果： ①BLM组大鼠肺泡炎及肺纤维化程度在不同时间点均明显高于同期NS组，差异明显（P<0.01）；②与BLM组相比，补阳还五汤各剂量组肺泡炎及肺纤维化程度有不同程度的改善（P<0.01 或 P<0.05）；③与NS组相比，BLM组肺组织TGF-β1，Smad3表达水平在不同时间点均明显增高（P<0.01），尤以第7天最为明显，并随时间推移有逐渐下降的趋势；④补阳还五汤各剂量组TGF-β1，Smad3表达水平与同期BLM组相有不同程度的降低（P<0.01或P<0.05），其中B组在7，14，28 d，C组在14，28 d下降较明显（P<0.01），A组与同期BLM组相比虽有所降低，但早期不如B，C组明显。结论：补阳还五汤可以明显改善模型大鼠肺组织肺泡炎和肺纤维化程度，抑制博莱霉素诱导的肺纤维化大鼠肺组织TGF-β1mRNA、Smad3mRNA的表达上调，从而减轻肺纤维化程度。

Abstract

Objective: To investigate the effects of Buyang Huanwu decoction on pathomorphology and the expression of transforming growth factor-β1(TGF-β1)mRNA and Smad3 mRNA in lung tissue of pulmonary fibrosis rats induced by bleomycin (BLM). Method: 144 healthy male SD rats were randomly divided into six groups:sham operation(NS) group

model(BLM)group

prednisone(P) group

Buyang Huanwu decoction high-dose

middle-dose

low-dose(A

C) group

24 rats in each group. Pulmonary fibrosis model was established by injection of BLM into tracheal intubation

NS group was intratracheally instilled with equal NS. The rats of group A

C were given Buyang Huanwu decoction at the dose of 18.48

9.24

4.62 g·kg-1

the rats of group P were given prednisone at the dose of 4.2 mg·kg-1

the rats of group NS and BLM were given NS at the dose of 10 mL·kg-1. 8 rats in each group were killed respectively on days 7

28 after intratracheal instillation. To measure alveolar catarrh and pulmonary fibrosis degrees in left lung dyed with HE and Masson

and TGF-β1mRNA

Smad3 mRNA in right lung tissue was assessed by RT-PCR. Result: ①Alveolar catarrh and pulmonary fibrosis degrees in group BLM were significantly higher than group NS(P<0.01);②The groups of Buyang Huanwu decoction could improve the alveolar catarrh and pulmonary fibrosis in varying degree by comparison with group BLM (P<0.01 or P<0.05);③At all time points

TGF-β1

Smad3mRNA expression in group BLM was higher than that in group NS(P<0.01)

on the 7th day the change was significant;④TGF-β1

Smad3 mRNA expression of the groups Buyang Huanwu decoction was lower than that in group BLM(P<0.01 or p<0.05)

TGF-β1

Smad3 mRNA expression of group B was lower than that in group BLM at all time points(P<0.01)

the levels of that in group C were significant lower than group BLM's on the 14th day and the 28th day(P<0.01)

the levels of group A's were lower than that in group BLM

but the differences were not more obvious on the 7th day than group B and C. Conclusion: Buyang Huanwu decoction could significantly alleviate alveolar catarrh and pulmonary fibrosis

efficiently regulate expression levels of TGF-β1

Smad3 mRNA in the lung tissues of pulmonary fibrosis rats induced by BLM

the degree of pulmonary fibrosis can be reduced.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Medicated Serum of Bupleuri Radix Regulates HFL1 Apoptosis and Fibroblast-myofibroblast Transition via Smad3/Rheb Axis

Effect of Kangxianling Decoction on TGF-β₁/Smad Signal Pathway in Renal Fibrosis Rats

Effect of Buyang Huanwu Decoction and Its Active Fractions Such as Alkaloid and Glycoside on TXA and PGI of Arterial Thrombosis Model in Rats

Effect of Buyang Huanwu Decoction and Its Alkaloid and Glycoside on Anticoagulative System Arterial Thrombosis Rat

Proteinuria and Transforming Growth Factor β1 in Rat Model of Glomerulosclerosis and the Intervention by Compound of Huoxue Bushen

Related Author

LI Da

SHEN Xiao

WU Quhui

ZHENG Lei

ZHAO Silin

JIN Zhaohui

XIAO Xuefei

FAN Fuyuan

Related Institution

The First Hospital of Hunan University of Chinese Medicine

Hunan University of Chinese Medicine

Shanghai University of Traditional Chinese Medicine (TCM)

Shuguang Hospital Affiliated to Shanghai University of TCM

College of Traditional Chinese Medicine,Capital Medical University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰