Objective: To observe effects of Fuzi Lizhong decoction on content of ANP

expression of pGC mRNA and gastrointestinal dynamics in rats with spleen Yang deficiency syndrome. Method: Hundred clean grade Wistar rats were randomly divided into control group

model group

Fuzi Lizhong decoction low-dose group

middle-dose group and high-dose group 5 groups with 20 rats in each group. The control group was given ordinary diet while model group was administrated by incising brown adipose tissue(BAT)

high fatty diet and cold enviroment stimulating. From the first day after incised BAT

model group was given high fatty diet for 3 weeks

and low-dose group

middle-dose group and high-dose group were ig respectively with Fuzi Lizhong decoction (10

20

40 g·kg-1) based on model group

once a day for 3 weeks. On the first day after 3 weeks the activities of jejunum muscle was observed andcolon was collected to examine content of ANP by ELISA and expression of pGC mRNA by RT-PCR. Result: Compared with the control group

in the model group

content of ANP and expression of pGC mRNA reduced(P<0.05 or P<0.01)

frequency of slow wave reduced significantly(P<0.01)while occurrence frequency of fast wave

maximum amplitude

spike burst per cluster increased(P<0.05 or P<0.01)

both amplitude of longitudinal muscle contraction and amplitude of circular muscle contraction enhanced significantly(P<0.01). Compared with the model group

in high-dose group

content of ANP and expression of pGC mRNA increased(P<0.05 or P<0.01).in middle-dose group frequency of slow wave increased(P<0.05) while occurrence frequency of fast wave

maximum amplitude

spike burst per cluster reduced(P<0.05 or P<0.01)

in high-dose group frequency of slow wave increased significantly(P<0.01)while occurrence frequency of fast wave

maximum amplitude

spike burst per cluster reduced significantly(P<0.01)

in middle-dose group amplitude of longitudinal muscle contraction reduced(P<0.05)while in high-dose group both amplitude of longitudinal muscle contraction and amplitude of circular muscle contraction reduced significantly(P<0.01). Conclusion: Fuzi Lizhong decoction could increase content of ANP and expression of pGC mRNA to restore gastrointestinal dynamics

meantime

regulate the reabsorption of water and NaCl

which perhaps is one of its mechanisms of improving symptoms of diarrhea.