Objective: To explore the effect of recombinant melittin on the glioma C6 cells and the expression of vascular endothelial growth factor (VEGF)

vascular endothelial growth factor receptor-1 (Flt-1) and matrix metalloproteinase-9 (MMP-9) in the cells. Method: Glioma C6 cells were separated into control group and 3 treatment groups

then 20

50

80 mg·L-1 recombinant melittin was given. MTT method was used to evaluate the inhibitory effects of recombinant melittin with different doses after 24

48

72 h on glioma C6 cell growth;the proteins expression of VEGF

Flt-1 and MMP-9 were determined by Western blot analysis. Result: Compared with control group

no significant change was found on glioma C6 cell growth after treated with recombinant melttin for 24 h in each treatment groups

but significantly inhibition of the glioma C6 cells proliferation was observed after treated with recombinant melttin for 48 h and 72 h in each groups (P<0.01 or P<0.05). The assay of Western blot showed that the proteins expression of VEGF

Flt-1 and MMP-9 in glioma C6 cells induced by recombinant melittin were significantly down-regulated when the cells were treated with 50

80 mg·L-1 recombinant melittin for 72 h. Conclusion: Recombinant melittin can inhibit the glioma C6 cell growth and the expression of VEGF

Flt-1 and MMP-9 in the cells

so recombinant melittin maybe can inhibit the growth of glioma by affect expression of VEGF

Flt-1 and MMP-9.