Optimization of Extraction Process of Airtight Chinese Medicine Composite Dressings

CAI Wen-zhi; PAN Yong-yi; LIU Zhi-gang; XIE Wen-hong; LI Li-li; LIU Qiang; CAI Bao-ta

doi:10.11653/syfj201324028

您当前的位置：

首页 >

文章列表页 >

Optimization of Extraction Process of Airtight Chinese Medicine Composite Dressings

更新时间：2024-01-04

- Optimization of Extraction Process of Airtight Chinese Medicine Composite Dressings
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 24, Pages: 28-31(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj201324028
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
CAI Wen-zhi, PAN Yong-yi, LIU Zhi-gang, et al. Optimization of Extraction Process of Airtight Chinese Medicine Composite Dressings[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 28-31.
DOI：

CAI Wen-zhi, PAN Yong-yi, LIU Zhi-gang, et al. Optimization of Extraction Process of Airtight Chinese Medicine Composite Dressings[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 28-31. DOI： 10.11653/syfj201324028.

摘要

目的：优选密闭性中药复合敷料的提取工艺。方法：以人参皂苷Rg1，Rb1含量和浸膏得率为评价指标，通过单因素试验优选提取次数，利用正交试验考察乙醇体积分数、乙醇用量、提取时间对密闭性中药复合敷料提取工艺的影响。采用HPLC测定人参皂苷Rg1，Rb1含量，流动相水（A）-乙腈（B）梯度洗脱（0~10 min，81% A；10~38 min，81%~79% A；38~90 min，79%~66% A），检测波长203 nm。结果：各因素对提取工艺的影响顺序为乙醇体积分数>提取时间>乙醇用量。最佳提取工艺条件为加8倍量70%乙醇提取2次，每次2 h；平均浸膏得率25.45%，人参皂苷Rg1，Rb1平均质量浓度分别为0.46，0.37 g·L-1。结论：建立的含量测定方法准确、重复性好，优选的提取工艺稳定、合理、可行，为民间外伤验方的二次开发提供参考。

Abstract

Objective: To optimize extraction process of airtight Chinese medicine composite dressings. Method: With contents of ginsenoside Rg1 and Rb1

extract yield as evaluation indexes

extraction times was optimized by single factor test

orthogonal design was adopted to optimize extraction technology of airtight Chinese medicine composite dressings by taking the amount and content of ethanol

extraction time as factors.Contents of ginsenoside Rg1 and Rb1 were determined by HPLC

mobile phase of water(A)-acetonitrile(B) with a gradient elution program(0-10 min

81% A;10-38 min

81%-79% A;38-90 min

79%-66% A)

detection wavelength 203 nm. Result: Effect of each factor on extraction technology was in order of ethanol concentration>extraction time>ethanol amount.Optimum extraction process were as follows:extracted 2 times with 8-fold the amount of 70% ethanol

2 hours per time;Average extract yield was 25.45%

average concentration of ginsenoside Rg1 and Rb1 were 0.46

0.37 g·L-1. Conclusion: This established method was accurate and reproducible

optimized extraction process was stable

reasonable and practicable

this study could provide a reference for secondary development of clinical prescriptions.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Extraction Process of Fufang Yungui Granules by Orthogonal Design

Optimization of Extraction Technology for Compound Yangshen Yizhi Tablet

Optimization of Extraction Technology for Main Components from Flower of

Optimization of Extraction Process of Xiaoer Pingchuan Qutan Granule by Orthogonal Design

Optimization of Water Extraction Process for Zhizichi Decoction

Related Author

ZHANG Xia

WANG Rong

YANG Jian-hong

HOU Yan-hui

LIU Yu

LIU Lian-xin

LI Su-ting

SUN Ting-ting

Related Institution

Changchun University of Chinese Medicine

School of Pharmacy, Liaoning University of Traditional Chinese Medicine (TCM)

Guangxi Scientific Research Center of TCM, School of Pharmacy, Guangxi University of Chinese Medicine

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰