Simultaneous Determination of Coumarin and Sesquiterpene Lactone Content of Active Ingredients in Chloranthus Species by HPLC

LUO Gang; MA Xing-xia; LUO Yong-ming; YIN Xiao-ying

doi:10.11653/syfj2014010047

您当前的位置：

首页 >

文章列表页 >

Simultaneous Determination of Coumarin and Sesquiterpene Lactone Content of Active Ingredients in Chloranthus Species by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Coumarin and Sesquiterpene Lactone Content of Active Ingredients in Chloranthus Species by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 1, Pages: 47-50(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2014010047
  CLC：
- Published：2014
- 稿件说明：
移动端阅览
LUO Gang, MA Xing-xia, LUO Yong-ming, et al. Simultaneous Determination of Coumarin and Sesquiterpene Lactone Content of Active Ingredients in Chloranthus Species by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 47-50.
DOI：

LUO Gang, MA Xing-xia, LUO Yong-ming, et al. Simultaneous Determination of Coumarin and Sesquiterpene Lactone Content of Active Ingredients in Chloranthus Species by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 47-50. DOI： 10.11653/syfj2014010047.

摘要

目的：建立同时测定金粟兰属植物中香豆素类成分异嗪吡啶及倍半萜内酯类成分长尾粗木叶内酯和党参内酯3种成分含量的高效液相色谱方法。方法：采用Hypersil C18柱（4.6 mm×250 mm，5 μm），乙腈-0.1%磷酸水溶液为流动相梯度洗脱，柱温30℃，检测波长230 nm，流速1.0 mL·min-1。结果：异嗪吡啶、长尾粗木叶内酯、党参内酯线性范围分别为2~100，2~100，4~200 ng，在上述范围内3种成分峰面积与进样量呈良好线性关系，相关系数r均>0.999，上述3种成分的加样回收率分别为96.1%（RSD 2.3%），94.4%（RSD 2.0%），94.2%（RSD 1.9%）；异嗪吡啶在多穗金粟兰中含量较高，最高含量为18 μg·g-1，长尾粗木叶内酯和党参内酯在丝穗金粟兰中含量较高，分别为2.4，1.2 μg·g-1。结论：该方法简便快速，分离效果好，重复性好，可为金粟兰属植物的质量控制提供重要参考。

Abstract

Objective: To develop an HPLC method for the simultaneous determination of three constituents about isofraxidin

lasianthuslactone and codonolactone. Method: The separation was performed on a Hypersil C18 column (4.6 mm×250 mm

5 μm) eluted with acetonitle and 0.1% phosphoric acid solution as mobile phase in a linear gradient mode. The flow rate was kept at 1 mL·min-1and the column temperature was set to 30℃

the UV detector wavelength was set at 230 nm. Result: The linear ranges were 2-100 ng for isofraxidin

2-100 ng for lasianthuslactone

4-200 ng for codonolactone

and the three components had a good linear relationship (r>0.999). Result of three components recovery tests were 96.1% (RSD 2.3%)

94.4% (RSD 2.0%)

94.2% (RSD 1.9%). The content of isofraxidin is more in Chloranhus multistachy

it is 18 μg·g-1;the codonolactone and lasianthuslactone were higher in C. fortumei

the content was 2.4

1.2 μg·g-1. Conclusion: Through the validation

the method is proved to be sensitive

accurate

repeatable

and can be used for quality control of C. plants.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Correlation Analysis Between Microbial Community Changes and Medicinal Quality Formation During Processing of Angelicae Dahuricae Radix

Establishment of Cryosectioning Method and Histochemical Localization Analysis of Volatile Oil and Coumarin Components of Ferula ferulaeoides

Analysis of Chemical Constituents in Violae Herba and Investigation of Its Antioxidant Activity in Vitro

Chemical Constituents from Stems of Zanthoxylum bungeanum

Establishment of Near Infrared Quantitative Analysis Model for Four Components in Cinnamomi Cortex

Related Author

CHEN Xiaoyan

ZHU Xinglong

GAN Qingxia

WANG Jiahao

AN Guangqin

WU Qinghua

PEI Jin

MA Yuntong

Related Institution

School of Pharmacy，State Key Laboratory of Southwest Chinese Medicine Resources， Chengdu University of Traditional Chinese Medicine

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization，Ministry of Education， School of Pharmacy，Shihezi University

Institute of Materia Medica，Shandong First Medical University & Shandong Academy of Medical Sciences，Ji′nan

School of Medicine and Life Sciences, University of Ji' nan, Shandong Academy of Medical Sciences

Institute of Materia Medica, Shandong Academy of Medical Sciences, Key Laboratory for Biotech-Drugs Ministry of Health, Key Laboratory for Rare & Uncommon Diseases of Shandong Province

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰