UPLC-MS-MS Analysis of Aflatoxins in  by Immunoaffinity Column Cleanup

CHEN Si-ying; ZHU Di; WANG Yong-lin; LAN Bo; XIONG Di-fei-fei; WANG Ai-min

doi:10.11653/syfj2014010051

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UPLC-MS-MS Analysis of Aflatoxins in by Immunoaffinity Column Cleanup

更新时间：2024-01-04

- UPLC-MS-MS Analysis of Aflatoxins in by Immunoaffinity Column Cleanup
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 1, Pages: 51-55(2014)
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- DOI：10.11653/syfj2014010051
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- Published：2014
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CHEN Si-ying, ZHU Di, WANG Yong-lin, et al. UPLC-MS-MS Analysis of Aflatoxins in by Immunoaffinity Column Cleanup[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 51-55.
DOI：

CHEN Si-ying, ZHU Di, WANG Yong-lin, et al. UPLC-MS-MS Analysis of Aflatoxins in by Immunoaffinity Column Cleanup[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 51-55. DOI： 10.11653/syfj2014010051.

摘要

目的：研究建立了免疫亲和柱净化UPLC-MS-MS测定天麻药材中黄曲霉毒素B1，B2，G1，G2的含量测定方法。方法：采用甲醇溶液超声提取、玻璃纤维滤纸滤过、黄曲霉毒素亲和免疫柱净化的方法对天麻药材样品进行处理，以超高液相色谱-三重四级杆串联质谱仪（UPLC-MS-MS）对黄曲霉毒素B1，B2，G1，G2含量进行研究并测定。结果：被测定的4种黄曲霉毒素分别在选定的范围内线性关系良好，精密度、重复性、准确度均良好，利用标准参考物质对本方法的验证结果良好，采用所建立的方法对30批不同产地天麻药材中黄曲霉毒素B1，B2，G1，G2含量进行了测定，结果均未检出。结论：该方法科学、可行、方便、快速，适用于对天麻药材中黄曲霉毒素B1，B2，G1，G2进行含量测定。

Abstract

Objective: The research aimed to establish a UPLC-MS-MS method for the determination of aflatoxin B1

G1 and G2 in Gastrodia elata. Method: The samples

G. elata herbs

were extracted by ultrasonic with methanol

filtered by glass fiber filter paper and purified by immuno affinity columns (IAC). Aflatoxin B1

G1 and G2 in G. elata were researched by UPLC-MS-MS. Result: The linear relationships of aflatoxin B1

G1 and G2were good in the scopes of selected concentration. The precision

repeatability and the accuracy were all satisfactory. There was a good result in using the reference material to validate this method. The method is used to determine the concentrations of the four aflatoxins in 30 batches of G. elata herbs and none were detected. Conclusion: The method is scientific

convenient and rapid to take. It can be applied into the determination of aflatoxin B1

G1 and G2 in G. elata herbs. And it provides objective references to help to evaluate the quality of G. elata herbs accurately and comprehensively.

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Related Institution

Hunan University of traditional Chinese medicine

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