HPLC Fingerprint of

HUANG Ping; LI Wen-jing; HE Bi-ting

doi:10.11653/syfj2014010066

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HPLC Fingerprint of

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- HPLC Fingerprint of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 1, Pages: 66-69(2014)
- 作者机构：
- 作者简介：
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- DOI：10.11653/syfj2014010066
  CLC：
- Published：2014
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HUANG Ping, LI Wen-jing, HE Bi-ting. HPLC Fingerprint of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 66-69.
DOI：

HUANG Ping, LI Wen-jing, HE Bi-ting. HPLC Fingerprint of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 66-69. DOI： 10.11653/syfj2014010066.

摘要

目的：建立毛鸡骨草药材的指纹图谱，为控制和评价药材质量提供依据。方法：用高效液相色谱法，Lichrospher C18色谱柱（4.6 mm×250 mm，5 μm），流动相甲醇-0.1%三氟乙酸（35：65），流速0.8 mL·min-1，检测波长272 nm，柱温室温。测定10批毛鸡骨草药材的指纹图谱，并进行相似度比较。结果：建立了毛鸡骨草药材的HPLC指纹图谱，确定9个共有峰，利用对照品指认其中4个峰。10批毛鸡骨草药材的相似度>0.98。结论：建立的方法快速、准确，为评价毛鸡骨草药材的质量提供参考。

Abstract

Objective: To establish a HPLC fingerprint analysis method for the quality control of Abrus mollis. Method: A HPLC method was adopted. Chromatographic assay was performed on a Lichrospher C18column (4.6 mm×250 mm

5 μm) at room temperature with methanol-0.1% TFA (35:65) as the mobile phase. The flow rate was 0.8 mL·min-1 and the detection wavelength was set at 272 nm. 10 batches samples of A. mollis were determined for similarity comparison. Result: A HPLC fingerprint analysis method of A. mollis was established. 9 common peaks were assigned

and 4 peaks of them were confirmed by comparing with reference standards. Similarity of 10 batches of A. mollis was more than 0.98. Conclusion: This method was rapid

accurate and reproducible

it could provide a reference for quality evaluation and control of A. mollis.

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