Simultaneous Determination of Peoniflorin, Costunolide, Dehydrocostus Lactone in Compound Berberine Tablets by HPLC

LIU Xiao-zhe; ZHANG Dong-fang

doi:10.11653/syfj2014010070

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Simultaneous Determination of Peoniflorin, Costunolide, Dehydrocostus Lactone in Compound Berberine Tablets by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Peoniflorin, Costunolide, Dehydrocostus Lactone in Compound Berberine Tablets by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 1, Pages: 70-73(2014)
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- DOI：10.11653/syfj2014010070
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- Published：2014
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LIU Xiao-zhe, ZHANG Dong-fang. Simultaneous Determination of Peoniflorin, Costunolide, Dehydrocostus Lactone in Compound Berberine Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 70-73.
DOI：

LIU Xiao-zhe, ZHANG Dong-fang. Simultaneous Determination of Peoniflorin, Costunolide, Dehydrocostus Lactone in Compound Berberine Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 70-73. DOI： 10.11653/syfj2014010070.

摘要

目的：同时测定复方黄连素片中芍药苷、木香烃内酯、去氢木香内酯3种成分的含量。方法：采用高效液相色谱法，C18柱，以乙腈（A）-0.2%的磷酸溶液（B）为流动相进行梯度洗脱，流速1.0 mL·min-1，检测波长225 nm，柱温30℃。结果：芍药苷在0.180 9~3.618 μg线性关系良好（r=0.999 8），平均加样回收率为99.0%，RSD 0.94%；木香烃内酯在0.129 85~2.597 μg线性关系良好（r=0.999 7），平均加样回收率为98.4%，RSD 1.62%；去氢木香内酯在0.120 8~2.416 μg线性关系良好（r=0.999 6），平均加样回收率为97.9%，RSD 1.21%。结论：该方法简便、准确，可用于测定复方黄连素片的含量。

Abstract

Objective: To establish a method for simultaneous determining peoniflorin

costunolide and dehydrocostus lactone in compound berberine tablets. Method: Three components were separated by C18 chromatographic column with a gradient mobile phase consisting of acetonitrile and 0.2% phosphoric acid solution at a flow rate of 1.0 mL·min-1

detection wavelength was 225 nm

and column temperature of 30℃. Result: The linear ranges of peoniflorin

costunolide

and dehydrocostus lactone fell within the ranges of 0.180 9-3.618

0.129 85-2.597

0.120 8-2.416 μg

respectively. The three components showed good linear correlations (r=0.999 8

0.999 7

0.999 6).The recoveries were 99.0% for peoniflorin

98.4% for costunolide

97.9% for dehydrocostus lactone

respectively. The relative standard deviations were 0.94%

1.62% and 1.21%. Conclusion: The method is simple and accurate. It can be used for quality control of compound berberine tablets.

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