Determination of Paeniflorin Content in Niuhuang Jiangya Pills by HPLC-MS-MS

LIN Jing; CHEN Tong-kai

doi:10.11653/syfj2014010084

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Determination of Paeniflorin Content in Niuhuang Jiangya Pills by HPLC-MS-MS

更新时间：2024-01-04

- Determination of Paeniflorin Content in Niuhuang Jiangya Pills by HPLC-MS-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 1, Pages: 84-86(2014)
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- DOI：10.11653/syfj2014010084
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- Published：2014
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LIN Jing, CHEN Tong-kai. Determination of Paeniflorin Content in Niuhuang Jiangya Pills by HPLC-MS-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 84-86.
DOI：

LIN Jing, CHEN Tong-kai. Determination of Paeniflorin Content in Niuhuang Jiangya Pills by HPLC-MS-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 84-86. DOI： 10.11653/syfj2014010084.

摘要

目的：建立HPLC-MS-MS法测定牛黄降压丸中芍药苷的含量。方法：采用HPLC-MS-MS法对牛黄降压丸中芍药苷进行含量测定，选用Agilent 20RBA×SB-C18反相柱（2.1 mm×30 mm，3.5 μm）为色谱柱，流动相为甲醇-5 mmol·L-1甲酸铵（75：25），流速0.4 mL·min-1，柱温25℃。质谱条件：电喷雾离子源（ESI），检测方式为多离子反应监测（MRM），负离子模式，用于定量分析的离子为m/z 525~449。结果：芍药苷的线性范围为20~2 000 mg·L-1（r=1.000 0）；样品平均加样回收率为99.56%，RSD 0.86%。结论：该方法操作简便，快速，准确度好，能有效控制牛黄降压丸的质量。

Abstract

Objective: To establish a HPLC-MS-MS method for the determination of paeoniflorin content in Niuhuang Jiangya pills. Method: Using a Agilent 20RBA×SB-C18 column (2.1 mm×30 mm

3.5 μm)

the mobile phase was consisted of methanol-water solution(75:25)

with 5 mmol ammonium formate solvent mixture that prepared for the LC conditioning. The flow rate was 0.4 mL·min-1

column temperature was 25℃. Using gas-auxiliary electrospray ionization source. Paeoniflorin (MRM m/z 525~449) were detected by the LC-MS. Result: The linear range was 20-2 000 mg·L-1

r=1.000 0.The average recovery of paeoniflorin was 99.56% with RSD of 0.86%. Conclusion: This method is simple

efficient and accurate

which can be used for the quality control of paeoniflorin in Niuhuang Jiangya pills.

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