Objective: To investigate the inhibitory effects of total flavonoids from the leaves of Carya cathayensis and their four monomer compounds

namely pinostrobin chalcone

pinocembrin

chrysin and wogonin on angiotensinⅡ(AngⅡ)-induced proliferation and migration in rat vascular smooth muscle cells (VSMC). Method: VSMC were primary cultured by tissue sticking method. VSMC proliferation was induced by 100 nmol·L-1 AngⅡ for 12 h after treatment of serum starvation at the initial density of 2.0×104/mL. VSMC migration was induced by 100 nmol·L-1 AngⅡ for 12 h at the initial density of 6.0×104/mL. Both of them were treated with 12.5-62.5 mg·L-1 total flavonoids

10-50 mg·L-1 pinostrobin chalcone

pinocembrin

chrysin and wogonin for 12 h. Cell proliferation was measured by direct counting the cells and cell migration was determined using the wound healing assay

respectively. Result: 12.5 mg·L-1 total flavonoids significantly inhibited AngⅡ-induced VSMC proliferation in a does dependent manner while 50 mg·L-1pinostrobin chalcone

chrysin and wogonin slightly inhibited AngⅡ-induced VSMC proliferation. 12.5 mg·L-1 total flavonoids

10 mg·L-1 pinostrobin chalcone

pinocembrin

chrysin and wogonin significantly inhibited AngⅡ-induced VSMC migration in a does dependent manner. Among them

the total flavonoids

chrysin and wogonin showed a higher inhibitory effect compared with those of pinocembrin and pinostrobin chalcone. Conclusion: The total flavonoids from the leaves of C. cathayensis can effectively inhibit AngⅡ-induced VSMC proliferation and migration

while their four monomer compounds present better suppressive activities on AngⅡ-induced migration than those of proliferation in VSMC.