Objective: To study the effect of Bushen Yangxue prescription

on expression of B-cell lymphoma/leukemia-2(Bcl-2) and Bcl-2 associated X protein(Bax) protein in ovaries of mice with immune premature ovarian failure(POF). Method: BALB/c female mice model of POF was established by multiple sites subcutaneous injection of zona pellucida 3 of mice

and then the modeled mice were treated with low

middle and high dosage Bushen Yangxue prescription

and positive control medicine of Estradio Valerate. Follicle-stimulating (FSH)

17-estradiol (E2)

lactogenic hormone (LH) were detected by radioimmunoassay method. Expression levels of Bcl-2 and Bax protein in mice ovarian were detected by western blot method. Result: Compared to Estradio Valerate group

FSH

E2

LH of low

middle and high dosage Bushen Yangxue prescription group were improved. Compared to the black control group

the expression of Bcl-2 protein of model group decreased obviously and the expression of Bax protein of model group increased obviously

Bcl-2/Bax ratio of model group decreased(P<0.05).Compared to the model group

the expression of Bcl-2 protein of high-dosage Bushen Yangxue prescription group and Estradio Valerate group increased obviously(P<0.01);the expression of Bcl-2 protein of low and middle dosage Bushen Yangxue prescription group increased obviously(P<0.05)

the expression of Bax protein of different dosage decreased obviously(P<0.05). All the data showed a dose-response relationship to some degree in expression of Bcl-2 protein within low

middle and high dosage Bushen Yangxue prescription group. Conclusion: It is indicated that rapid apoptosis of follicle may result in premature ovarian failure

and Bushen Yangxue prescription can improve the function of ovaries by up-regulating the expression of Bcl-2 protein and down-regulating the expression of Bax protein in ovarian.