Effects of Cinobufacini Injections on Proliferation and P-YAP Expression of Hepatocar HepG-2 Cells

TIAN Li-li; HOU Li; GAO Shan; CUI Xiao-nan

doi:10.11653/syfj2014010153

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Effects of Cinobufacini Injections on Proliferation and P-YAP Expression of Hepatocar HepG-2 Cells

更新时间：2024-01-04

- Effects of Cinobufacini Injections on Proliferation and P-YAP Expression of Hepatocar HepG-2 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 1, Pages: 153-156(2014)
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- DOI：10.11653/syfj2014010153
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- Published：2014
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TIAN Li-li, HOU Li, GAO Shan, et al. Effects of Cinobufacini Injections on Proliferation and P-YAP Expression of Hepatocar HepG-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 153-156.
DOI：

TIAN Li-li, HOU Li, GAO Shan, et al. Effects of Cinobufacini Injections on Proliferation and P-YAP Expression of Hepatocar HepG-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 153-156. DOI： 10.11653/syfj2014010153.

摘要

目的：探讨华蟾素注射液对人肝癌HepG-2细胞增殖及磷酸化YAP蛋白（P-YAP）表达的影响。方法：将实验分为对照组，华蟾素注射液0.105，0.21，0.42 mg·L-1组；采用CCK8观察华蟾素注射液对HepG-2细胞增殖的影响；采用流式细胞术检测华蟾素注射液对HepG-2细胞凋亡及周期的影响；采用蛋白免疫印迹法（Western blot法）观察华蟾素注射液对HepG-2细胞中P-YAP蛋白表达的影响。结果：华蟾素注射液能抑制HepG-2细胞增殖，促进凋亡，且呈时间和剂量依赖性（P<0.05）；阻滞HepG-2细胞于S期，呈剂量依赖性。药物作用1 h，P-YAP蛋白表达上调，呈剂量依赖性（P<0.05）；药物作用12 h，P-YAP蛋白的表达无明显变化，与对照组无显著差异；药物作用24 h，P-YAP蛋白表达下调（P<0.05），呈剂量依赖性。结论：华蟾素注射液能抑制人肝癌HepG-2细胞增殖，上调P-YAP蛋白表达可能是华蟾素注射液早期抗肝癌药效机制之一。

Abstract

Objective: To investigate the effects of Cinobufacini injections on the proliferation and protein phosphorylation

YAP(P-YAP) expression of hepatocar HepG-2 cells(HHC). Method: Cell proliferation was assessed by cell counting kit-8(CCK8) assay.Cell apoptosis and cycles were shown through flow cytometry method(FCM). The expressions of P-YAP protein were analyzed by Western blot. Result: Cinobufacini injections significantly inhibited HepG-2 cell proliferation

promoted apoptosis(P<0.05)in dose-and time-dependent ways

blocked cycles at the S-phase in a dose-dependent way;increased the expression of P-YAP in a dose-dependent way after 1 h (P<0.05)

had no significant influence on the expression of P-YAP after 12 h

decreased the expression of P-YAP in a dose-dependent way after 24 h(P<0.05). Conclusion: Cinobufacini injections can inhibit proliferation

increased P-YAP expression may be one of its anti-tumor mechanisms.

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