Objective: To investigate the anti-inflammatory and asthma-relieving effects of total flavones from Nigella glandulifera (TFNG). Method: KM mice

SD rats and New Zealand rabbits were selected as experiment animal

randomly devided into saline control group

positive control group

TFNG low dosage and high dosage groups (mouse 100

400 mg·kg-1

rat 71.5

286 mg·kg-1). The mice external ear swelling induced by dimethyl benzene and rats cotton ball granuloma experiments were employed to evaluate the anti-inflammatory effect;asthma experiment induced by spraying the whole mice

isolated lung perfusion and isolated ileum smooth muscle experiments in guinea pigs were employed to evaluate the asthma-relieving effect. Result: TFNG 100

400

71.5 mg·kg-1 could significantly inhibit dimethyl benzene-induced mice ear swelling and TFNG 286 mg·kg-1 could siginificatey inbibit cotton pellet granuloma in rats(P<0.01);the low and high dosage (100

400 mg·kg-1) TFNG could significantly prolong the mice asthmatic response latency (P<0.01);the low

middle and high dosage TFNG (3

6

12 g·L-1) could increase the perfusion of the isolated guinea pig lung

reduce the smooth muscle contraction of isolated guinea pig ileum significantly (P<0.01). Conclusion: TFNG has some anti-inflammatory and asthma-relieving effects.