Optimization of Separation and Purification Technology for Icariin and Icarisid Ⅱ from Epimedii Folium by Macroporous Adsorption Resin

REN Gui-you; LIU Hai-ping; WANG Shuang; LI Qiong; ZENG Rui

doi:10.11653/syfj2014020005

您当前的位置：

首页 >

文章列表页 >

Optimization of Separation and Purification Technology for Icariin and Icarisid Ⅱ from Epimedii Folium by Macroporous Adsorption Resin

更新时间：2024-01-04

- Optimization of Separation and Purification Technology for Icariin and Icarisid Ⅱ from Epimedii Folium by Macroporous Adsorption Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 2, Pages: 5-7(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2014020005
  CLC：
- Published：2014
- 稿件说明：
移动端阅览
REN Gui-you, LIU Hai-ping, WANG Shuang, et al. Optimization of Separation and Purification Technology for Icariin and Icarisid Ⅱ from Epimedii Folium by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 5-7.
DOI：

REN Gui-you, LIU Hai-ping, WANG Shuang, et al. Optimization of Separation and Purification Technology for Icariin and Icarisid Ⅱ from Epimedii Folium by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 5-7. DOI： 10.11653/syfj2014020005.

摘要

目的：建立大孔树脂同时分离纯化淫羊藿中淫羊藿苷和淫羊藿次苷Ⅱ的工艺条件。方法：以淫羊藿苷和淫羊藿次苷Ⅱ的吸附容量、洗脱率为评价指标，通过静态吸附-洗脱试验筛选大孔树脂型号，采用单因素试验优选淫羊藿苷和淫羊藿次苷Ⅱ的吸附和洗脱条件。结果：选用DM301型大孔吸附树脂，最佳吸附条件为上样液质量浓度7.45 g·L-1，上样液流速3 BV·h-1；淫羊藿苷洗脱条件为加45%乙醇4 BV以1.5 BV·h-1的流速进行洗脱；淫羊藿次苷Ⅱ洗脱条件为加60%乙醇5 BV以1.5 BV·h-1的流速进行洗脱；淫羊藿苷、淫羊藿次苷Ⅱ纯度分别达56.23%，67.41%。结论：建立的工艺条件可同时分离纯化淫羊藿苷和淫羊藿次苷Ⅱ，且稳定性好、收率较高、生产成本低，适宜于规模化生产推广。

Abstract

Objective: To optimize separation and purification technology of icariin and icariside Ⅱ from Epimedii Folium by macroporous adsorption resin. Method: Taking adsorption capacity and desorption rate of icariin and icariside Ⅱ as indexes

macroporous resin models were screened by static adsorption-elution test

single factor tests were adopted to investigate adsorption and elution conditions. Result: DM301 macroporous resin was the most suitable one for purification of icariin and icariside Ⅱ

its optimum adsorption conditions were as follows:the concentration of sample solution 7.45 g·L-1

sample flow rate 3 BV·h-1;Elution conditions of icariin was eluting with 4 BV 45% ethanol at 1.5 BV·h-1;Elution conditions of icatiside Ⅱ was eluting with 5 BV 60% ethanol at 1.5 BV·h-1;Under these conditions

purities of icariin and icariside Ⅱ were 56.23% and 67.41%

respectively. Conclusion: These established process conditions could be adopted to separate and purify icariin and icariside Ⅱ at the same time with good stability

high yield and low production cost

which were suitable for industrial production.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Separation and Antitumor Activity Investigation of Active Constituents from Toad Skin

Effect of Active Components of Epimedii Folium in Promoting Proliferation and Differentiation of Osteoblasts

Intestinal Absorption Ingredients in Epimedii Folium and Investigation of Their Absorption Characteristics by Everted Intestinal Sac Method

Comparison of Efficiency of Compressed Pieces and Traditional Pieces of Epimedii Folium in Different Decoction Times

Optimization of Ethanol Extraction Technology for Total Flavonoids in Roots of and Screening of Its Type of Macroporous Resin

Related Author

CAO Wei

LIU Cong-yan

CHEN Yan

YUAN Ling

WANG Ying

GE Xun

CAO Guang-shang

SUN Hong-sheng

Related Institution

Graduate School，Beijing University of Chinese Medicine

The First Affiliated Hospital，Baotou Medical College，Inner Mongolia University of Science and Technology

Peking University Health Science Center

Japan Friendship Hospital

Guizhou University of Traditional Chinese Medicine（TCM）

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰