YANG Pei-pei, ZHOU Han-yu, CONG Xiao-dong, et al. Rapid Determination of Total Glycosides in Countercurrent Extracting Process of Gardeniae Fructus by Near-Infrared Spectroscopy[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 36-38.
DOI:
YANG Pei-pei, ZHOU Han-yu, CONG Xiao-dong, et al. Rapid Determination of Total Glycosides in Countercurrent Extracting Process of Gardeniae Fructus by Near-Infrared Spectroscopy[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 36-38. DOI: 10.11653/syfj2014020036.
Rapid Determination of Total Glycosides in Countercurrent Extracting Process of Gardeniae Fructus by Near-Infrared Spectroscopy
Objective: To develope a near-infrared quantitative method for rapid determination of total glycosides in countercurrent extracting process of Gardeniae Fructus. Method: UV was used as a reference method to determine the content of total glycosides in Gardeniae Fructus
a rapid on-line determination method of total geniposides was established by NIR transmission method and partial least squares method.Sampling method for transmission acquisition
collection interval 4 000-10 000 cm-1
resolution 8.0 cm-1
numner of scans 64 times
empty door attenuation
gain of 1
temperature at (25±2) ℃
relative humidity 45%-50%. Result: Correlation coefficients and calibration root-mean-square error of calibration model for total glycosides in countercurrent extract of Gardeniae Fructus were 0.993 and 0.179
respectively.By external validation
prodiction root-mean-square error of calibration model was 0.145
correlatiaon coefficient of regression equation between the actual value and the predicted value was 0.991. Conclusion: NIR could be used as a kind of accurate
rapid and on-line detection method for determination of active ingredients in extracting process of traditional Chinese medicine.
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Related Institution
Henan Key Laboratory for Modern Research on Zhongjing's Herbal Formulae,Academy of Chinese Medical Sciences,Henan University of Chinese Medicine
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