Objective: To establish a method to determine contents of astragaloside Ⅰ

astragaloside Ⅲ and astragaloside IV from Astragali Radix

then analyzed influence of honey-processed for artragaloside content. Method: HPLC-ELSD was adopted

Thermo Syncronis C18 column(4.6 mm×250 mm

5 μm)

mobile phase consisting of acetonitrile-0.3% formic acid using a gradient elution

flow rate of 0.8 mL·min-1 and column temperature at 25 ℃

drifttube temprature of ELSD parameters was 110 ℃

volume flow rate of nitrogen was 3.0 L·min-1. Result: Astragaloside Ⅰ

astragaloside Ⅲ and astragaloside IV showed a good linear relationship in the range of 0.804-8.04

0.784-7.84

0.406-4.06 μg

the average recoveries of them were 98.3%

99.3%

98.9% with RSD of 2.46%

1.89%

2.38%

respectively.Contents of astragaloside Ⅰ

astragaloside Ⅲ and astragaloside IV from Astragali Radix in different places were 0.301-0.527

0.186-0.303

0.128-0.178 mg·g-1

respectively. Conclusion: This established method was simple and reliable

methodology validation met requirements.Contents of astragaloside Ⅰ and astragaloside Ⅲ from processed Astragali Radix were higher than raw materials

but astragaloside IV was opposite

indicating that honey-processed had an effect on the content of saponins.