HPLC-DAD-ELSD Simultaneous Determination of Four Triterpenoids in Rhizoma Alismatis

QIU Jian-fang; LIN Jing; XU Wen; HUANG Ming-qing; ZHAO Wan-li; LUO Fen-rong; LI Xiao-yan; WU Shui-sheng

doi:10.11653/syfj2014020042

您当前的位置：

首页 >

文章列表页 >

HPLC-DAD-ELSD Simultaneous Determination of Four Triterpenoids in Rhizoma Alismatis

更新时间：2024-01-04

- HPLC-DAD-ELSD Simultaneous Determination of Four Triterpenoids in Rhizoma Alismatis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 2, Pages: 42-46(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2014020042
  CLC：
- Published：2014
- 稿件说明：
移动端阅览
QIU Jian-fang, LIN Jing, XU Wen, et al. HPLC-DAD-ELSD Simultaneous Determination of Four Triterpenoids in Rhizoma Alismatis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 42-46.
DOI：

QIU Jian-fang, LIN Jing, XU Wen, et al. HPLC-DAD-ELSD Simultaneous Determination of Four Triterpenoids in Rhizoma Alismatis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 42-46. DOI： 10.11653/syfj2014020042.

摘要

目的：建立泽泻药材中23-乙酰泽泻醇C、泽泻醇A、泽泻醇B、23-乙酰泽泻醇B 4个三萜类成分的HPLC-DAD-ELSD同时测定的定量分析方法。方法：采用Ultimate XB-C18色谱柱（4.6 mm×150 mm，5 μm），流动相乙腈-水（65：35），流速1.0 mL·min-1，二极管阵列检测器检测波长245 nm，蒸发光散射检测器的漂移管温度60 ℃，雾化器温度55 ℃，气体（氮气）压力20 psi，柱温30 ℃。结果： 23-乙酰泽泻醇C、泽泻醇A、泽泻醇B、23-乙酰泽泻醇B 4个成分的线性范围分别为1.869~29.90 mg·L-1（r=0.999 8），3.731~74.62 mg·L-1（r=0.999 7），8.653~138.4 mg·L-1（r=0.999 9），4.832~77.31 mg·L-1（r=0.999 5），平均加样回收率分别为98.78%（RSD 2.63%），98.05%（RSD 2.72%），98.26%（RSD 2.86%），97.65%（RSD 2.95%）。结论：建立的HPLC-DAD-ELSD定量分析方法简便、快捷、准确，可用于泽泻药材的多成分定量分析，为综合评价泽泻的质量提供一种新的技术手段。

Abstract

Objective: An analytical method was developed for simultaneously determining alisol C 23-acetate

alisol A

alisol B

alisol B 23-acetate four triterpenoids in the rhizoma of Alisma orientalis. Method: The assay was performed on a Ultimate XB-C18 column (4.6 mm×250 mm

5 μm) with the mobile phase consisting of acetonitrile-water (65:35) at a flow rate of 1.0 mL·min-1. The column temperature was at 30 ℃ and the optimum detection wavelength of DAD was set at 245 nm. The drift tube and atomizer temperature of ELSD were 60

55 ℃

with a carrier gas (N2) pressure of 20 psi. Result: The linear range of alisol C 23-acetate

Alisol A

alisol B and alisol B 23-acetate were 1.869-29.90 mg·L-1(r=0.999 8)

3.731-74.62 mg·L-1(r=0.999 7)

8.653-138.4 mg·L-1(r=0.999 9)

4.832-77.31 mg·L-1(r=0.999 5)

respeltively. The average recoveries (n=6) were 98.78%(RSD 2.63%)

98.05%(RSD 2.72%)

98.26%(RSD 2.86%) and 97.65%(RSD 2.95%) respectively. Conclusion: The HPLC method is simple

rapid and accurate. This HPLC-DAD-ELSD verified method can be used for quantitative analysis of multi-component of Rhizoma Alismatis and provides a novel approach for evaluation of the quality of Rhizoma Alismatis comprehensively.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Simultaneous Determination of Six Triterpenoids in Alismatis Rhizoma by UFLC

Discussion on Technical Characteristics of National Drug Standards for Traditional Chinese Medicine Dispensing Granules

Analysis on Quality Standard of Sennae Folium（Cassia angustifolia） Dispensing Granules Based on Standard Decoctions

Herbal Textual Research and Modern Research Progress of Ostreae Concha

Aanlysis on Quality Attribute Transfer Law of Liquid-solid Preparation of Shengxuebao

Related Author

LIU Yi-wen

XU Ming-tao

CHEN Shengjun

LI Song

GUO Kejia

ZHANG Yuntian

ZHOU Haiqin

PU Xianglan

Related Institution

Jiangyin Tianjiang Pharmaceutical Co. Ltd.，Jiangyin

Shandong Technical Innovation Center for Traditional Chinese Medicine Treatment of Respiratory Diseases，Shandong Hongjitang Pharmaceutical Group Co. Ltd.

Shandong Institute for Food and Drug Control

School of Pharmacy，State Key Laboratory of Southwestern Chinese Medicine Resources， Chengdu University of Traditional Chinese Medicine

Hunan Polytechnic of Environment and Biology

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰