SHANG Lei, OUYANG Zhen, ZHAO Ming, et al. HPLC-MS-MS Analysis and Determination of Alkaloids of Mori Folium[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 47-51.
DOI:
SHANG Lei, OUYANG Zhen, ZHAO Ming, et al. HPLC-MS-MS Analysis and Determination of Alkaloids of Mori Folium[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 47-51. DOI: 10.11653/syfj2014020047.
HPLC-MS-MS Analysis and Determination of Alkaloids of Mori Folium
Objective: To use liquid chromatography-mass spectrometry techniques for qualitative analysis of alkaloids of Mori Folium and determine the content of total alkaloids in alkaloids in Mori Folium by HPLC-UV with pre-column derivatization. Method: The chromatographic separation was performed on a HiQSiL C18 column (4.6 mm×250 mm
5 μm) using acetonitrile and 0.1% acetic acid aqueous solution under isocratic elution(50:50) at a flow rate of 0.8 mL·min-1.Mori Folium alkaloids was analyzed with 9-fluorenylmethyl chlorformate(FMOC-Cl) as the pre-column derivative agent. Detection wavelength was set at 254 nm with electrospray ionization source(ESI) and positive ion mode. And 1-deoxynojirimycin as the external standard to calculate mulberry leaf total alkaloid content. Result: Six alkaloid compounds were identified as DNJ
fagomine
3-epi-fagomine
DAB
calystegine B2 and 2-O-β-Glc-DAB.The content of 3 batches test samples were more than 75%. Conclusion: The method is sensitive
accurate
simple
and reliable. It can be used for quantitative analysis of Mori Folium alkaloids and preparation.
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College of Chinese Materia Medica, Beijing University of Chinese Medicine
College of Life Sciences, Beijing University of Chinese Medicine
Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine
Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine(TCM),School of Pharmacy,Henan University of Chinese Medicine