UPLC Fingerprint of  from Different Areas

HAN Yong-cheng; LIU Wei; CHEN Ning; CUI Yong-xia; HUANG Li-jie

doi:10.11653/syfj2014020067

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UPLC Fingerprint of from Different Areas

更新时间：2024-01-04

- UPLC Fingerprint of from Different Areas
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 2, Pages: 67-69(2014)
- 作者机构：
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- DOI：10.11653/syfj2014020067
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- Published：2014
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HAN Yong-cheng, LIU Wei, CHEN Ning, et al. UPLC Fingerprint of from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 67-69.
DOI：

HAN Yong-cheng, LIU Wei, CHEN Ning, et al. UPLC Fingerprint of from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 67-69. DOI： 10.11653/syfj2014020067.

摘要

目的：建立不同产地金银花药材的超高效液相特征性指纹图谱，为有效控制和科学评价金银花药材整体质量提供依据。方法：采用Agilent C18 色谱柱（2.1 mm×50 mm，1.8 μm），流动相乙腈-0.2%磷酸水，以0.4 mL·min-1的流速进行梯度洗脱，检测波长238 nm，柱温30 ℃。结果：在21 min内得到金银花药材的指纹图谱，对其中5个色谱峰进行了初步归属，并对14批药材样品进行了分析，其相似度为0.915~0.987。结论： UPLC指纹图谱方法较HPLC大大缩短了分析时间，可用于金银花药材的质量评价。

Abstract

Objective: To develop an UPLC method of determining the characteristic chromatographic profile of Lonicera japonica for controlling the drug quality quickly and accurately. Method: The analysis was performed on an Agilent C18 column(2.1 mm×50 mm

1.8 μm) with acetonitrile-0.2% phosphoric acid as mobile phase in gradient elution mode. The flow rate was 0.4 mL·min-1 and the injection volume was 2.0 μL with the detection wavelength of 238 nm.The column temperature was set at 30 ℃. Result: The fingerprint of Lonicera japonica was obtained within 21 min and five peaks in the fingerprint were designed.14 samples were analyzedand the similarities were 0.915-0.987. Conclusion: The UPLC system enjoys the merits of a higher speed

sensitivity

and resolution

it can be used to control the quality of L. japonica.

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