Study on HPLC Fingerprint of Arnebiae Radix Smashing Tissue Extraction with Ethanol

WANG Hong; PENG Bing; LI Ping; HAN Xu-yang; ZENG Zu-ping

doi:10.11653/syfj2014020074

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Study on HPLC Fingerprint of Arnebiae Radix Smashing Tissue Extraction with Ethanol

更新时间：2024-01-04

- Study on HPLC Fingerprint of Arnebiae Radix Smashing Tissue Extraction with Ethanol
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 2, Pages: 74-77(2014)
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- DOI：10.11653/syfj2014020074
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- Published：2014
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WANG Hong, PENG Bing, LI Ping, et al. Study on HPLC Fingerprint of Arnebiae Radix Smashing Tissue Extraction with Ethanol[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 74-77.
DOI：

WANG Hong, PENG Bing, LI Ping, et al. Study on HPLC Fingerprint of Arnebiae Radix Smashing Tissue Extraction with Ethanol[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 74-77. DOI： 10.11653/syfj2014020074.

摘要

目的：建立紫草闪式提取醇提物的HPLC指纹图谱，全面完整地反映其内在化学信息，为紫草醇提物的质量控制提供依据。方法：采用高效液相色谱法，色谱柱为Kromasil 100-5 C18（4.6 mm×250 mm，5 μm），以水-甲酸（300：0.5）-乙腈为流动相进行梯度洗脱，检测波长275 nm，流速1 mL·min -1，柱温25 ℃。结果：建立了10批紫草闪式提取醇提物的HPLC指纹色谱图，获得了13个共有峰，并指认了其中的3个色谱峰，4，6，10号分别被指认为左旋紫草素、乙酰紫草素和β，β’-二甲基丙烯酰阿卡宁。结论：该方法简便，精密度、重复性和稳定性良好，能有效控制紫草醇提物的质量，为进一步药效研究、制剂研发提供质量保证。

Abstract

Objective: To establish the HPLC fingerprint of Arnebiae Radix smashing tissue extraction with ethanol and reflect the chemical information comprehensively and provide evidence for quality control. Method: HPLC was used on a Kromasil 100-5 C18 (4.6 mm×250 mm

5 μm) by gradient elution with solvent system composed of water-formic acid (300:0.5)-acetonitrile

detection wavelength was 275 nm

the flow rate was 1 mL·min-1

the column temperature was set at 25 ℃. Result: HPLC fingerprint of Arnebiae Radix smashing tissue extraction with ethanol was established

which showed 13 characteristic peaks from 10 batches of crude drug. Among them

the No 4

10 peaks were respectively identified as shikonin

acetylshikonin

and β

β'-dimethylacryl alkannin. Conclusion: The method is simple and has good precision

reproducibility and stability

and can be applied to the quality control the Arnebiae Radix smashing tissue extraction with ethanol

also provide a quality assurance for further pharmacological studies and preparation research.

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