GAO Qiao-ying, LIU Da-quan, WU Shang-wei, et al. Qingre Jiedu Prescription Treating Septic through Down-Regulating AQPs Expression in Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 133-136.
DOI:
GAO Qiao-ying, LIU Da-quan, WU Shang-wei, et al. Qingre Jiedu Prescription Treating Septic through Down-Regulating AQPs Expression in Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 133-136. DOI: 10.11653/syfj2014020133.
Qingre Jiedu Prescription Treating Septic through Down-Regulating AQPs Expression in Rats
Objective: To investigate the mechanism of Qingre Jiedu prescription in treatment septic. Method: The septic model was induced by cecal ligation and puncture. The experimental rats were divided into five groups;control group
septic model group
Qingre Jiedu prescription treated group (24
12
6 g·kg-1). All animals were treaded via gastrogavage with distilled water and Qingre Jiedu prescription once a day for 3 days. The survival state was observed. The bacterial populations in experimental sepsis were determined by bacterial culture. And aquaporins(AQP) 2 and AQP8 were detected by western blot. Result: The fatality rate in model group was the highest (56%)
meanwhile all bacteria in ascites
lymph nodes and bloods were significantly higher than that in control group. For treaded group
the mortality and the serum biochemical parameter in the high dose group were significantly decreased. The AQP2 and AQP8 expression levels in all treated groups were decreased compared with that in model group
with a dose-dependent manner. In the high dose group
the number of pathogens in lymph nodes and blood was significantly lower than that in model group. Conclusion: Qingre Jiedu prescription can decrease bacterial translocation by reducing expression of AQPs to increase excreta.
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