HU Yue-qiang, TANG Nong, WU Lin, et al. Effect of Qingre Huayu Granule on the Expression of Inositol Requiring Enzyme 1 after Focal Ischemic Preconditioning in Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 144-147.
DOI:
HU Yue-qiang, TANG Nong, WU Lin, et al. Effect of Qingre Huayu Granule on the Expression of Inositol Requiring Enzyme 1 after Focal Ischemic Preconditioning in Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 144-147. DOI: 10.11653/syfj2014020144.
Effect of Qingre Huayu Granule on the Expression of Inositol Requiring Enzyme 1 after Focal Ischemic Preconditioning in Rats
Objective: To observe the effect of the Qingre Huayu granule (QRHY) on the inositol requiring enzyme 1(IRE1) mRNA and protein after focal ischemic preconditioning in rats. Method: Eighty healthy male SD rats were randomly divided into four groups:sham-operation group
middle cerebral artery occlusion (MCAO) group
brain ischemia preconditioning(BIP) group and QRHY group.Each group was further divided into 4 subgroups according to ischemia/reperfusion(I/R) duration of 12 h
1
2
3 d.The BIP models was established and 14 mL·kg-1 QRHY granule solution was ig given twice everyday for 3 days after preconditioning. But the MCAO and BIP group was ig given same amount physiological saline.The expression of IRE1 mRNA and protein was detected by Real time PCR and Western blot. Result: The expression both of IRE1 mRNA and its protein all increased and reached the peak at 24 h
then decreased continuously(P<0.05). BIP could increase their expression (P<0.05 or P<0.01). QRHY treatment could further induce its expression compared with BIP group(P<0.05 or P<0.01). Conclusion: BIP could upregulate the expression of IRE1 after focal ischemic injury in rats. QRHY could augment the expression of IRE1 induced by BIP.