Objective: To observe the effect of Huatan Tongluo therapy on gene expression of brain microvascular endothelial cells in rats with acute cerebral infarction after recombinant tissue-type plasminogen(rt-PA) thrombolysis

and provide experimental basis for preventive and therapeutict effects of Huatan Tongluo on ischemia-reperfusion injury induced by acute cerebral infarction after thrombolysis. Method: Two hundred and fouty healthy SD rats were randomly divided into six groups

sham operation group

model group

rt-PA group

rt-PA combined with Huatan Tongluo low dose group

rt-PA combined with Huatan Tongluo middle dose group

rt-PA combined with Huatan Tongluo high dose group. The middle cerebral artery occlusion(MCAO) rat model was prepared by autologous blood clots. rt-PA group and rt-PA combined with Huatan Tongluo groups used rt-PA thrombolytic therapy after thrombosis. The later were also given low

medium

high dose(3.6

7.2

14.4 g·kg-1) Huatan Tongluo prescription by gavage two times a day. Then observed the expression of occluding

claudin-1

ZO-1 in rat cortex and hippocampus by RT-PCR at 6

24

72 h and 7 days respectively. Result: In different phases of every experimental group

the expression of occluding

claudin-1

ZO-1 in different brain tissue were highest at 6 h

minimum at 24 h

increased gradually at 72 h and 7 days

and the combined with Huatan Tonglow groups increased more obviously. The expression of occluding

claudin-1

ZO-1 in rt-PA

low

middle

high dose groups at 6 h were significantly higher than that at 24

72 h(P<0.05). Compared the model group at the same time

the expression of occluding

claudin-1 in middle dose and high dose groups of Chinese traditional medicine were significantly increasedin each phase (P<0.05). While the ZO-1 gene expression had the similar trends

but significant difference only existed at the 24

72 h

7 d (P<0.05). Conclusion: Huatan Tongluo combined with rt-PA therapy can regulate the expression of occluding

claudin-1

ZO-1 in brain microvascular endothelial cells. So it can protect the integration of blood brain barrier

and have protective effects on ischemia reperfusion injury induced by acute cerebral infarction after thrombolysis.