Optimization of Dilute Acid Ethanol Extraction Technology for 1-Deoxynojirimycin from Mori Folium by Response Surface Methodology

SU Nan; HE Xian-xia; FENG Qing; WU Xin-rong

doi:10.11653/syfj2014030013

您当前的位置：

首页 >

文章列表页 >

Optimization of Dilute Acid Ethanol Extraction Technology for 1-Deoxynojirimycin from Mori Folium by Response Surface Methodology

更新时间：2024-01-04

- Optimization of Dilute Acid Ethanol Extraction Technology for 1-Deoxynojirimycin from Mori Folium by Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 3, Pages: 13-16(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2014030013
  CLC：
- Published：2014
- 稿件说明：
移动端阅览
SU Nan, HE Xian-xia, FENG Qing, et al. Optimization of Dilute Acid Ethanol Extraction Technology for 1-Deoxynojirimycin from Mori Folium by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 13-16.
DOI：

SU Nan, HE Xian-xia, FENG Qing, et al. Optimization of Dilute Acid Ethanol Extraction Technology for 1-Deoxynojirimycin from Mori Folium by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 13-16. DOI： 10.11653/syfj2014030013.

摘要

目的：优化桑叶中1-脱氧野尻霉素（1-DNJ）的稀酸乙醇浸提工艺。方法：采用HPLC-ELSD测定1-DNJ含量，流动相乙腈-水（90：10），ELSD漂移管温度40 ℃，载气流速3.5×105 Pa。以1-DNJ提取率为指标，在单因素试验基础上，通过响应面分析法考察浸提时间、乙醇体积分数及pH对桑叶中1-DNJ提取工艺的影响，利用Design Expert 7.1.6分析软件对试验数据进行分析。结果：最佳工艺参数为乙醇体积分数66.53%，pH 6.66，浸提时间2.73 h；1-DNJ实际提取率0.194 6%，与理论量最优值0.1%相差较小。结论：建立的二次多项数学模型准确可靠，优选的提取工艺稳定可行，为桑叶资源的开发利用提供参考。

Abstract

Objective: To optimize dilute acid ethanol extraction technology of 1-deoxynojirimycin(1-DNJ) from Mori Folium. Method: HPLC-ELSD was adopted to determine the content of 1-DNJ with mobile phase of acetonitrile-water (90:10) and ELSD drift tube temperature of 40 ℃

flow rate of carrier gas was 3.5×105 Pa.With yield of 1-DNJ as index

based on single factors

response surface methodology was adopted to investigated effects of soaking time

ethanol concentration and pH on extraction technology of 1-DNJ

then test data were analyzed by Design Expert 7.1.6 software. Result: Optimum process conditions of 1-DNJ were as follows:ethanol concentration 66.53%

pH value of 6.66

soaking time 2.73 h;Under these conditions

yield of 1-DNJ was 0.194 6%

which had small difference between theoretical optimal value of 0.100%. Conclusion: This established quadratic multinomial model was accurate and reliable

optimized technology could provide a reference for development and utilization of Mori Folium resources.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Extraction Process and Mechanism of Active Ingredients of Mori Folium for Lowering Blood Glucose： A Review

Chemical Constituents and Insulin Resistance Targets in Different Parts of Morus alba by UPLC-Q-TOF-MS Combined with Network Pharmacology

Herbal Textual Research on Mori in Famous Classical Formulas

Mori Folium Improves Glucose and Lipid Metabolism Disorders in Rats with Type 2 Diabetes Mellitus by Regulating PI3K/Akt/PPARα/CPT-1 Pathway

Study on Frosting Quality Markers of Mori Folium Based on Component Characteristic

Related Author

XIE Jiamei

ZHANG Huilin

HUANG Yan

WANG Menglu

DU Yuhang

HE Changhao

AN Yongcheng

FU Wanxin

Related Institution

Beijing Research Institute of Chinese Medicine， Beijing University of Chinese Medicine

College of Life Sciences， Beijing University of Chinese Medicine

College of Chinese Materia Medica， Beijing University of Chinese Medicine

Laboratory of Supramolecular Mechanism and Mathematic-Physics Characterization for Traditional Chinese Medicine（TCM）， Hunan Key Laboratory of Druggability and Preparation Modification of TCM， School of Pharmacy， Hunan University of Chinese Medicine

School of Pharmacy，School of Food and Biological Engineering， Jiangsu University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰