Objective: To optimize extraction technology of Tangyushu granules. Method: With composite score of dry extract rate and extraction amounts of berberine hydrochloride and 2

3

5

4'-tetrahydroxystilbene-2-O-β-D-glucoside (stilbene glycoside) as index

orthogonal test was adopted to optimize water extraction technology of Tangyushu granules by taking the amount of water

extraction time and times as factors.Contents of berberine hydrochloride and stilbene glycoside were determined by HPLC. Result: Extraction times had significant influence on water extraction effect

but the amount of water and extraction time had not significant influence.Optimum extraction technology of Coptidis Rhizoma from Tangyushu granules was as follows:extracted three times with ten times the amount of water

one hour each time;Dregs of Coptidis Rhizoma and others pieces in the prescription were extracted three times with eight times the amount of water

one and a half hours each times;Average extracting amounts of stilbene glycoside and berberine hydrochloride were 48.40

5.032 mg·g-1

respectively. Conclusion: This optimized extraction process was stable and feasible

which could be extended to industrial production of Tangyushu granules.