Simultaneous Determination of Four Flavonoids in  by HPLC

DONG Lin; GUAN Wei-wei; SHENG Lin; WANG Jin-hui; LIU Ming-sheng

doi:10.11653/syfj2014030052

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Simultaneous Determination of Four Flavonoids in by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Four Flavonoids in by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 3, Pages: 52-55(2014)
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- DOI：10.11653/syfj2014030052
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- Published：2014
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DONG Lin, GUAN Wei-wei, SHENG Lin, et al. Simultaneous Determination of Four Flavonoids in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 52-55.
DOI：

DONG Lin, GUAN Wei-wei, SHENG Lin, et al. Simultaneous Determination of Four Flavonoids in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 52-55. DOI： 10.11653/syfj2014030052.

摘要

目的：建立同时测定裸花紫珠药材中木犀草素-7-O-β-D-吡喃葡萄糖苷、木犀草素、5，4’-二羟基-3，7，3’-三甲氧基黄酮和5-羟基-3，7，3’，4’-四甲氧基黄酮含量的反相高效液相色谱法。方法：采用HPLC，Venusil XBP-C18色谱柱（4.6 mm×200 mm，5 μm），流动相甲醇-体积分数为0.1%磷酸水系统，梯度洗脱，流速1.0 mL·min﹣1，柱温室温，检测波长350 nm。结果：在上述色谱条件下测得木犀草素-7-O-β-D-吡喃葡萄糖苷、木犀草素、5，4’-二羟基-3，7，3’-三甲氧基黄酮和5-羟基-3，7，3’，4’-四甲氧基黄酮的质量浓度分别在0.000 8~0.016，0.001 6~0.032，0.004~0.08，0.002 4~0.048 g·L-1 时与色谱峰面积之间线性关系良好；平均回收率为98.5%（RSD 1.7%），98.6%（RSD 1.6%），98.6%（RSD 1.4%），98.6%（RSD 1.7%）。结论：该方法精密度高，重复性好，可用于裸花紫珠药材中木犀草素-7-O-β-D-吡喃葡萄糖苷、木犀草素、5，4’-二羟基-3，7，3’-三甲氧基黄酮和5-羟基-3，7，3’，4’-四甲氧基黄酮的含量测定。

Abstract

Objective: To develop HPLC for the simultaneous determination of four flavonoids in Callicarpa nudiflora. Method: The separation was performed on a Venusil XBP-C18column(4.6 mm×200 mm

5 μm)

the analytes were separated efficiently using the mobile phase consisted of MeOH-H2O in a gradient program. The flow rate was maintained at 1.0 mL·min﹣1.The detective wavelength was set at 350 nm and the column temperature was 25 ℃. Result: There were good linear relationships between the concentrations and the peak area of luteolin-7-O-β-D-glucopyranoside

luteolin

4'-dihydroxy-3

3'-trimethoxyflavone and 5-hydroxy-3

4'-tetramethoxyflavone in the ranges of 0.000 8-0.016 g·L-1(r=0.999 1)

0.001-0.032 g·L-1(r=0.999 1)

0.004-0.08 g·L-1(r=0.999 2)

0.002 4-0.048 g·L-1 (r=0.999 1)

respectively. The recoveries were found to be 98.5% with RSD of 1.7%(n=9)

98.6% with RSD of 1.6%(n=9)

98.6% with RSD of 1.4%(n=9) and 98.6% with RSD of 1.7%(n=9)

respectively. Conclusion: The method is accurate and reproducible for determination of luteolin-7-O-β-D-glucopyranoside

luteolin

4'-dihydroxy-3

3'-trimethoxyflavone and 5-hydroxy-3

4'-tetramethoxyflavone in C. nudiflora.

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