Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method

XIN Min; LIU Xuan; ZHAN Xin; HUANG Yun; TANG Jin-tian; SHENG Jun

doi:10.11653/syfj2014030062

您当前的位置：

首页 >

文章列表页 >

Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method

更新时间：2024-01-04

- Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 3, Pages: 62-65(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2014030062
  CLC：
- Published：2014
- 稿件说明：
移动端阅览
XIN Min, LIU Xuan, ZHAN Xin, et al. Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 62-65.
DOI：

XIN Min, LIU Xuan, ZHAN Xin, et al. Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 62-65. DOI： 10.11653/syfj2014030062.

摘要

目的：比较苯酚-硫酸法与硫酸-紫外法在测定千两茶多糖含量上的差异。方法：千两茶粉经水提醇沉，Sevage法除蛋白后，采用苯酚-硫酸法和硫酸-紫外法测定茶多糖含量。结果：苯酚-硫酸法标准曲线方程为Y=4.755 0X-0.029 5（R2=0.990 4），硫酸-紫外法标准曲线方程为Y=4.770 0X-0.065 1（R2=0.993 1），均在0.02~0.1 g·L-1有良好的线性关系。所测茶多糖质量分数分别为2.00%，3.21%，其差异有统计学意义。结论：硫酸-紫外法作为一种多糖检测新手段，可简便测定多糖含量。

Abstract

Objective: To compare differences between phenol-sulfuric acid method and sulfuric acid-UV method to determine polysaccharides content in Qianliang tea. Method: The two methods were employed to determine content of polysaccharides in Qianliang tea after water extracting-alcohol precipitating and sevage assays. Result: The total sugar stand curve equation were Y=4.755 0X-0.029 5(R2=0.990 4)(by sulfuric acid-UV method) and Y=4.770 0X-0.065 1(R2=0.993 1)(by phenol-sulfuric acid method)

which showed a good linear relationship range from 0.02 to 0.1 g·L-1. The determination of phenol-sulfuric acid method and sulfuric acid-UV method were 2.00% and 3.21%

which was statistically significance. Conclusion: As a new method

sulfuric acid-UV method could be a stable and simple method to measure the polysaccharide.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰