Simultaneous Determination of Five Ingredients in Wuzi Yanzong Pill by HPLC

LIU Wei; ZHOU Jian-liang; CHEN Bi-lian; ZHU Ming

doi:10.11653/syfj2014030074

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Simultaneous Determination of Five Ingredients in Wuzi Yanzong Pill by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Five Ingredients in Wuzi Yanzong Pill by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 3, Pages: 74-78(2014)
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- DOI：10.11653/syfj2014030074
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- Published：2014
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LIU Wei, ZHOU Jian-liang, CHEN Bi-lian, et al. Simultaneous Determination of Five Ingredients in Wuzi Yanzong Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 74-78.
DOI：

LIU Wei, ZHOU Jian-liang, CHEN Bi-lian, et al. Simultaneous Determination of Five Ingredients in Wuzi Yanzong Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 74-78. DOI： 10.11653/syfj2014030074.

摘要

目的：建立五子衍宗丸中5种成分金丝桃苷、山柰素、五味子醇甲、五味子甲素和五味子乙素的含量测定方法。方法： Angilent Extend C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-甲醇（10：1）和0.4%磷酸，梯度洗脱柱温30 ℃，流速1.0 mL·min-1，检测波长250 nm（五味子醇甲、五味子甲素、五味子乙素），360 nm（金丝桃苷、山柰素）。结果：在该色谱条件下，金丝桃苷、山柰素、五味子醇甲、五味子甲素和五味子乙素分离良好，分别在4.824~482.4，4.358~87.16，1.854~185.4，5.610~112.2，3.850~77.00 ng呈良好的线性关系，加样回收率（n=9）依次为101.2%，98.9%，101.6%，101.3%，97.6%。结论：所建立的方法简便、准确、重复性好，可用于五子衍宗丸的质量控制。

Abstract

Objective: A method for simultaneous determination of five ingredients in Wuzi Yanzong pill was developed by reversed-phase high-performance liquid chromatography (HPLC). Method: HPLC was performed on Angilent Extend C18 column (4.6 mm×250 mm

5 μm) with a gradient elution system using methanol:ethylactate(10:1)-0.4% phosphoric acid at 30 ℃ and at a flow rate of 1.0 mL·min-1. The eluate was detected by wavelength of 250 nm for schisandrin

deoxyschizandrin

γ-schizandrin and 360 nm for hyperoside and kaempferide. Result: Hyperoside

kaempferide

schisandrin

deoxyschizandrin and γ-schizandrin were baseline separated and their linear ranges were 4.824-482.4

4.358-87.16

1.854-185.4

5.610-112.2

3.850-77.00 ng

respectively. The recovery of the five compounds (n=9) were 101.2%

98.9%

101.6%

101.3%

97.6%

respectively. Conclusion: The developed method is simple

accurate

and shows good precision and reproducibility. It is could be used for the quality control of Wuzi Yanzong pill.

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Related Author

LI Bin1

REN Hai-hua2

LI Min-fang1

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GUO Rong

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Related Institution

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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The First Clinical Medical College of Guangzhou University of Chinese Medicine

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