UPLC Fingerprint of

CHEN Ning; HAN Yong-cheng; LIU Wei; CUI Yong-xia; WU Ming-xia; WANG Li-guo; HUANG Li-jie

doi:10.11653/syfj2014030083

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UPLC Fingerprint of

更新时间：2024-01-04

- UPLC Fingerprint of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 3, Pages: 83-85(2014)
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- DOI：10.11653/syfj2014030083
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- Published：2014
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CHEN Ning, HAN Yong-cheng, LIU Wei, et al. UPLC Fingerprint of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 83-85.
DOI：

CHEN Ning, HAN Yong-cheng, LIU Wei, et al. UPLC Fingerprint of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 83-85. DOI： 10.11653/syfj2014030083.

摘要

目的：建立不同产地野菊花药材的超高效液相特征性指纹图谱，为全面有效控制和科学评价野菊花药材整体质量提供科学依据。方法：采用Agilent C18色谱柱（2.1 mm×50 mm，1.8 μm），流动相乙腈-0.1%磷酸水溶液，0.4 mL·min-1梯度洗脱，进样量1.0 μL，检测波长326 nm，柱温25 ℃。结果：在12 min内得到野菊花药材的指纹图谱，对其中5个色谱峰进行了初步归属，并对15批药材样品进行了分析，其相似度为0.871~0.985。结论：相对于HPLC指纹图谱，UPLC指纹图谱方法具有更好分离效率、更高灵敏度，并大大缩短了分析时间，可用于野菊花药材的质量控制。

Abstract

Objective: To develop an UPLC method of determining the characteristic chromatographic profile of Chrysanthemum indicum for controlling the drug quality quickly and accurately. Method: The analysis was performed on an Agilent C18 column (2.1 mm×50 mm

1.8 μm) with acetonitrile-0.1% phosphoric acid as mobile phase in gradient elution mode.The flow rate was 0.4 mL·min-1 and the injection volume was 1.0 μL with the detection wavelength of 326 nm.The column temperature was set at 25 ℃. Result: The fingerprint of C. indicum was obtained within 12 min

and five peaks in the fingerprint were designed. 15samples were analyzed

and the similarities were 0.871-0.985. Conclusion: The UPLC system enjoys the merits of a higher speed

sensitivity

and resolution

compared with the HPLC system. It can be used to control the quality of Chrysanthemum indicum.

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