Urinary Excretion of Daphnoretin and its Metabolite at Different Doses in Rats

GAO Jin-wei; WANG Xiao-bo; WANG Cai-fen; CUI Yu-qi; TONG Li-jin; SUN Li-xin

doi:10.11653/syfj2014030099

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Urinary Excretion of Daphnoretin and its Metabolite at Different Doses in Rats

更新时间：2024-01-04

- Urinary Excretion of Daphnoretin and its Metabolite at Different Doses in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 3, Pages: 99-103(2014)
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- DOI：10.11653/syfj2014030099
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- Published：2014
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GAO Jin-wei, WANG Xiao-bo, WANG Cai-fen, et al. Urinary Excretion of Daphnoretin and its Metabolite at Different Doses in Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 99-103.
DOI：

GAO Jin-wei, WANG Xiao-bo, WANG Cai-fen, et al. Urinary Excretion of Daphnoretin and its Metabolite at Different Doses in Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 99-103. DOI： 10.11653/syfj2014030099.

摘要

目的：研究不同剂量西瑞香素及其葡萄糖醛酸化代谢产物在大鼠尿中的排泄动力学。方法：将西瑞香素制成混悬液，按50，100，200 mg·kg-1给大鼠灌胃，于灌胃后不同时间收集尿液，用β-葡糖醛酸苷酶溶液处理尿液。采用高效液相色谱法测定尿液中西瑞香素及其葡萄糖醛酸化代谢产物的浓度。结果： 50，100，200 mg·kg-13种给药剂量时，累积经尿液排泄的原形药物量为（23.13±2.36），（41.74±2.02）和（80.01±3.21）μg，累积经尿液排泄的葡萄糖醛酸结合形药物为（37.51±4.51），（61.17±3.90）和（131.25±13.85）μg。结论：西瑞香素在大鼠尿液中葡萄糖醛酸化代谢产物的排泄量大于原形排泄量，原形及其葡萄糖醛酸化代谢产物的排泄呈现明显的剂量依赖性特征。

Abstract

Objective: To study the urinary excretion of daphnoretin and glucuronidated daphnoretin in rats. Method: Suspended in solution

daphnoretin was orally administered to rats at the dose of 50

100

200 mg·kg-1. At various time intervals

the urine was collected. The urine was treated with β-glucuronidase. The daphnoretin in urine was extracted by acetic ether.The organic phase was removed into the tubes and then dried in pressure blowing concentrator. The residue was dissolved in 200 μL of mobile phase. The solution of 20 μL was drawn and detected by high-perormance liquid chromatography. Result: The cumulative urine excretion of daphnoretin was (23.13±2.36)

(41.74±2.02) and (80.01±3.21) μg at the dose of 50

100

200 mg·kg-1

respectively. The cumulative urine excretion of glucuronidated daphnoretin was (37.51±4.51)

(61.17±3.90) and (131.25±13.85) μg at the dose of 50

100

200 mg·kg-1

respectively. Conclusion: The cumulative urine excretion of glucuronidated daphnoretin is more than cumulative urine excretion of daphnoretin. The daphnoretin and glucuronidated daphnoretin are excreted in the manner of linear dose-dependent.

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