Effect of Ethyl Acetate Extract from on Cholesterol Accumulation and SREBP-1 Expression in THP-1-derived Lipid-loaded Cells

KUANG Shuang-yu; LI Yi; KUANG Wen-ding; LIU Ta-si

doi:10.11653/syfj2014030137

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Effect of Ethyl Acetate Extract from on Cholesterol Accumulation and SREBP-1 Expression in THP-1-derived Lipid-loaded Cells

更新时间：2024-01-04

- Effect of Ethyl Acetate Extract from on Cholesterol Accumulation and SREBP-1 Expression in THP-1-derived Lipid-loaded Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 3, Pages: 137-140(2014)
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- DOI：10.11653/syfj2014030137
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- Published：2014
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KUANG Shuang-yu, LI Yi, KUANG Wen-ding, et al. Effect of Ethyl Acetate Extract from on Cholesterol Accumulation and SREBP-1 Expression in THP-1-derived Lipid-loaded Cells[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 137-140.
DOI：

KUANG Shuang-yu, LI Yi, KUANG Wen-ding, et al. Effect of Ethyl Acetate Extract from on Cholesterol Accumulation and SREBP-1 Expression in THP-1-derived Lipid-loaded Cells[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 137-140. DOI： 10.11653/syfj2014030137.

摘要

目的：观察菝葜乙酸乙酯提取物对人单核/巨噬细胞系（THP-1）源性荷脂细胞胆固醇蓄积的影响，并初步探讨类固醇调节因子结合蛋白-1（SREBP-1）蛋白表达在其中的作用。方法：以THP-1源性荷脂细胞为模型，洛伐他汀（80 mg·L-1）做阳性对照，菝葜乙酸乙酯提取物不同浓度（0，40，80，160 mg·L-1）作用于细胞24 h以及80 mg·L-1作用于细胞不同时间（0，12，24，48 h），高效液相色谱法测定细胞内总胆固醇（TC），游离胆固醇（FC），胆固醇酯（CE）的含量，油红O染色观察菝葜乙酸乙酯提取物对THP-1源性荷脂细胞中脂滴形成的影响，Western blot印迹法检测细胞内SREBP-1蛋白表达变化。结果：菝葜乙酸乙酯提取物明显降低THP-1源性荷脂细胞中脂滴含量，细胞内TC，FC，CE水平呈现一定的剂量和时间依赖性下降趋势，同时 SREBP-1蛋白表达上调。结论：菝葜乙酸乙酯提取物能引起THP-1源性荷脂细胞TC，FC，CE含量下降，且这种作用与SREBP-1蛋白表达上调有一定关系。

Abstract

Objective: To observe the effect of ethyl acetate extract from Smilax china on the cholesterol metabolism in monocyte/macrophage cell line(THP-1)-derived lipid-loaded cells

and to investigate the effect of the expression of sterol regulatory element binding protein(SREBP-1) and caveolin-1 in this process. Method: The ethyl acetate extract with different density (0

160 mg·L-1) were used to detect the level of total cholesterol(TC)

free cholesterol(FC)

cholesterol ester(CE) by using high performance liquid phase analytic process and the expression of SREBP-1 by Western blot at different time (0

48 h) in THP-1 derived lipid-loaded cells. Result: The ethyl acetate extract from S.china had some effects on THP-1-derived lipid-loaded cells

the lipid droplet density decreased in cells and the level of TC

CE was decreased with a dose and time-dependent manner. The expression of SREBP-1 was upregulated. Conclusion: The ethyl acetate extract from S.china can decreased the level of TC

CE in THP-1 derived lipid-loaded cells

and it is contributed to the up-regulation of SREBP-1 expression at some degree.

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