Objective: To evaluate the effect of Cujing Tang (CJT) on mouse semen routine

sex hormone and sperm apoptosis. Method: Fourty mice were used to build oligozoospermia and asthenozoospermia model with cyclophosphamide (CTX) 60 mg·kg-1·d-1 for 5 days

and randomly divided into model group

CJT low dose treatment group

middle dose treatment group and high dose treatment group

with 10 rats in each group. In addition

a blank control group was set up. The blank control group and model group received 0.5 mL normal saline per mouse by gavage. After modeling

low

middle and high dose treatment group were given with 2.5

5

2.10 g·kg-1 CJT by gavage for 30 days. The quality of mice sperm were detected by CASA

serum sex hormone was detected by ELISA and sperm apoptosis was detected by flow cytometry. Result: After modeling the sperm density

vitality and viability of model group were significantly reduced (P<0.05). CJT could significantly improve mice sperm density

vitality and viability (P<0.05)

and high dose goup had no significant difference compared with blank group. follicle-stimulating hormone(FSH) and prolactin(PRL) had no significant difference in all groups. luteotropic hormone(LH)

testosterone(T) and estradial(E2) of model group were significantly higher than other groups (P<0.05). The LH

T and E2 were reduced in all CJT group

and especially high dose group had no significant difference compared with blank group. Sperm apoptosis of model group was significantly higher than that of blank group (P<0.05). The apoptosis rate were decreased in all CJT group

especially high dose group

which had no significant difference compared with blank group. Conclusion: CJT can increase sperm density

motility and viability

repaire testicular pathological injury and decrease the apoptosis rate of testicular spermatogenic cell.