DAI Bing, LI Mei, GAO Peng, et al. Influence of ZTC1+1-Ⅱ Clarifier on Purification Effect of Extracts of Total Saponins from Polygalae Radix[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 12-15.
DOI:
DAI Bing, LI Mei, GAO Peng, et al. Influence of ZTC1+1-Ⅱ Clarifier on Purification Effect of Extracts of Total Saponins from Polygalae Radix[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 12-15. DOI: 10.11653/syfj2014040012.
Influence of ZTC1+1-Ⅱ Clarifier on Purification Effect of Extracts of Total Saponins from Polygalae Radix
Objective: To optimize clarification process of extracts of total saponins from Polygalae Radix with ZTC1+1-Ⅱ clarifier. Method: With solids removal rate and retention rate of tenuifolin as indexes
based on single factor tests
orthogonal test was adopted to optimize clarification process of extracts of total saponins from Polygalae Radix by taking the concentration of extracts
reaction temperature
stirring speed and dosage of ZTC1+1-Ⅱ clarifier as factors.HPLC was adopted to determine the content of tenuifolin with mobile phase of methanol-0.05% phosphoric acid(65:35) and detection wavelength of 210 nm. Result: Optimal clarification process was concentrated extracts to 0.125 g·mL-1
added component B with dosage of 0.8 g·L-1 by insulation stirring
then added component A with dosage of 0.4 g·L-1 after two hours
bath temperature of two components were 80 ℃
stirring speeds were 90 r·min-1
insulation time and stirring time were 20 and 10 min
respectively;Retention rate of tenuifolin and solids removal rate were 84.56% and 21.97%
respectively. Conclusion: ZTC1+1-Ⅱ natural clarify agents had good clarification effect on extracts of total saponins from Polygalae Radix.