Effects of Different Decoction Ways on Content of Berberine in Chaishao Changning Capsules

LIU Yao; ZHANG Yong-ping; TANG Yun; WANG Wan-lian; XU Jian; HU Wen-yan; YE Zu-guang

doi:10.11653/syfj2014040019

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Effects of Different Decoction Ways on Content of Berberine in Chaishao Changning Capsules

更新时间：2024-01-04

- Effects of Different Decoction Ways on Content of Berberine in Chaishao Changning Capsules
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 4, Pages: 19-21(2014)
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- DOI：10.11653/syfj2014040019
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- Published：2014
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LIU Yao, ZHANG Yong-ping, TANG Yun, et al. Effects of Different Decoction Ways on Content of Berberine in Chaishao Changning Capsules[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 19-21.
DOI：

LIU Yao, ZHANG Yong-ping, TANG Yun, et al. Effects of Different Decoction Ways on Content of Berberine in Chaishao Changning Capsules[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 19-21. DOI： 10.11653/syfj2014040019.

摘要

目的：建立柴芍肠宁胶囊中盐酸小檗碱的含量测定方法，考察不同煎煮方式对盐酸小檗碱含量的影响。方法：采用HPLC测定盐酸小檗碱含量，DIKMA ODS-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.1%磷酸（每100 mL加十二烷基磺酸钠0.1 g）（50：50），检测波长345 nm。考察黄连与其他药味合煎或分煎时对盐酸小檗碱含量的影响。结果：盐酸小檗碱在2.4~24 mg·L-1线性关系良好（r=1），平均回收率101.14%，RSD 2.48%。分煎、合煎样品中盐酸小檗碱平均质量分数分别为0.128 2，0.078 6 mg·g-1，RSD依次为1.02%，1.25%。结论：黄连与其他药味分煎时盐酸小檗碱含量显著高于合煎，为柴芍肠宁胶囊的临床应用提供参考。

Abstract

Objective: To establish a method for determining the content of berberine in Chaishao Changning capsules and investigate effects of different decoction ways on the content of berberine. Method: HPLC was adopted to determine the content of berberine with DIKMA ODS-C18 column (4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile-0.1% phosphoric acid(per 100 mL plus sodium dodecyl sulfate 0.1 g)(50:50) and detection wavelength at 345 nm.Effects of mixed decoction and single decoction of Coptidis Rhizoma with other herbs on the content of berberine were investigated. Result: Berberine had a goog linear relationship with 2.4-24 mg·L-1 with r=1

the average recovery was 101.14% with RSD of 2.48%.Average contents of berberine from decoction and separate decoction samples were 0.128 2

0.078 6 mg·g-1 with RSD of 1.02%

1.25%

respectively. Conclusion: The content of berberine from mixed decoction samples was significantly higher than single decoction samples

but it should be made conclusion through clinical trials.

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Related Institution

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