Determination of Two Anthraquinones in  by HPLC

LIU Yan-qun; YIN Wen-jie; ZUO Lin; MAN Qi-qian; LV Qing-tao; JIANG Hai-qiang; GONG Li-li

doi:10.11653/syfj2014040042

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Determination of Two Anthraquinones in by HPLC

更新时间：2024-01-04

- Determination of Two Anthraquinones in by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 4, Pages: 42-44(2014)
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- DOI：10.11653/syfj2014040042
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- Published：2014
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LIU Yan-qun, YIN Wen-jie, ZUO Lin, et al. Determination of Two Anthraquinones in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 42-44.
DOI：

LIU Yan-qun, YIN Wen-jie, ZUO Lin, et al. Determination of Two Anthraquinones in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 42-44. DOI： 10.11653/syfj2014040042.

摘要

目的：建立白花蛇舌草药材中2种蒽醌化合物2-羟基-3-甲氧基-7-甲基蒽醌（蒽醌Ⅰ）和2-羟基-1-甲氧基蒽醌（蒽醌Ⅱ）含量测定的方法。方法：采用Eclipse XDB-C18柱（4.6 mm×250 mm，5 μm），以甲醇-水（60：40）等度洗脱，流速1.0 mL·min-1，检测波长272 nm，柱温25 ℃。结果：蒽醌Ⅰ和蒽醌Ⅱ分别在4.0~160 mg·L-1（r=0.999 6）和4.0~160 mg·L-1（r=0.999 5）线性关系良好，平均加样回收率（n=9）分别为99.27%，99.08%，供试品溶液在24 h内稳定。结论：该方法精密度良好，准确度高，适合于白花蛇舌草药材中2-羟基-3-甲氧基-7-甲基蒽醌和2-羟基-1-甲氧基蒽醌含量的测定。

Abstract

Objective: To develop an HPLC method for quantitative determination of 2-hydroxy-3-methoxy-7-methylanthraquinone (anthraquinone Ⅰ) and 2-hydroxy-1-methoxyanthraquinone (anthraqui-none Ⅱ) in Hedyotis diffusa. Method: A Eclipse XDB-C18 column (4.6 mm×250 mm

5 μm) was adopted with a mobile phase of methanol-water (60：40) at the flow rate of 1.0 mL·min-1

the detection wavelength was set at 272 nm and the column temperature was 25 ℃. Result: The calibration curves were linear in the ranges of 4.0-160 mg·L-1 (r=0.999 6) for anthraquinone Ⅰ and 4.0-160 mg·L-1(r=0.999 5) for anthraquinone Ⅱ. The average recoveries (n=9) of anthraquinone Ⅰ and anthraquinone Ⅱ were 99.27% and 99.08%

respectively. The sample solution was steady within 24 h. Conclusion: The method is precise and accurate for determination of 2-hydroxy-3-methoxy-7-methylanthraquinone and 2-hydroxy-1-methoxyanthraquinone in H. diffusa.

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Related Institution

School of Chinese Materia Medica, Beijing University of Chinese Medicine

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