Objective: To establish a HPLC fingerprint of Gardenia(Gardenia jasminoids) decoction pieces which is made with Zhangbang method

so as to provide a reference for its quality control. Method: Chromatographic separation was carried out by an Agilent Zorbax Eclipse XBD-C18 column(4.6 mm×250 mm

5 μm)and gradient elution was performed by mobile phase containing acetonitrile and water. The flow rate was 1.0 mL·min-1

the column temperature was maintained at 30 ℃. The detector was set at 238 nm(0-20 min)

440 nm(20-40 min). Result: A consistent Zhangbang Gardenia decoction pieces HPLC fingerprint pattern containing 9 common peaks was obtained. We also compared the similarities between the fingerprints of 11 batches decoction pieces from different areas and the standard one we have established. Similarities ranges from 0.495 to 0.952. Conclusion: Similarities between the 10 batches Zhangbang Gardenia decoction pieces we obtained from Zhangshu Tianqitang decoction factory was certainly good. Similarities between the 11 batches from different areas but all were made with the same method and the standard fingerprint we had established for Zhangbang method is quite uneven.