Identification of TLC and Determination of HPLC on Juglandis Semen

ZHANG Xue; XU Jia-jia; PAN Ying-ni; QI Wen; PANG Jun-zhu; LIU Xiao-qiu

doi:10.11653/syfj2014040062

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Identification of TLC and Determination of HPLC on Juglandis Semen

更新时间：2024-01-04

- Identification of TLC and Determination of HPLC on Juglandis Semen
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 4, Pages: 62-64(2014)
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- DOI：10.11653/syfj2014040062
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- Published：2014
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ZHANG Xue, XU Jia-jia, PAN Ying-ni, et al. Identification of TLC and Determination of HPLC on Juglandis Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 62-64.
DOI：

ZHANG Xue, XU Jia-jia, PAN Ying-ni, et al. Identification of TLC and Determination of HPLC on Juglandis Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 62-64. DOI： 10.11653/syfj2014040062.

摘要

目的：建立核桃仁TLC鉴别和HPLC含量测定方法。方法：采用TLC法对核桃仁中glansreginins A进行定性鉴别并采用HPLC法对其进行含量测定。Diamonsil C18色谱柱（4.6 mm×200 mm，5 μm），流速0.8 mL·min-1，检测波长265 nm，流动相乙腈-1%冰醋酸，梯度洗脱。结果：在TLC鉴别中，glansreginins A斑点清晰、分离效果好；在HPLC测定中，glansreginins A在24.26~242.6 mg·L-1线性关系良好，平均回收率为96.4%（RSD 2.7%）。结论：所建立的TLC和HPLC法操作简便、专属性强且重复性好，可作为核桃仁定性、定量的质量控制方法。

Abstract

Objective: To establish the methods of identification of TLC and determination of HPLC in the Juglandis Semen. Method: Glansreginins A in Juglandis Semen was identified by TLC method and the content was determined by HPLC method. The chromatographic column was Diamonsil C18(4.6 mm×200 mm

5 μm). The flow rate was 0.8 mL·min-1

the detection wavelength was set at 265 nm. A gradient elution of acetonitrile-water containing 1% acetic acid was used. Result: The TLC method had high sensitivity and showed clear spots of glansreginins A;the glansreginins A showed a good linearity within the range of 24.26-242.6 mg·L-1

and the average recovery rate was 96.4%(RSD 2.7%). Conclusion: The methods are simple

reliable and repeatable. They can be used for the quality control of Juglandis Semen.

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