Objective: To establish the measurement method of the multi-component with a single-marker(QAMS) of Shuganjianpi pill

and verify the accuracy

adaptability

geasibility of QAMS in the quality control. Method: Shuganjianpi pill was used as the research object and the hesperidin was used as the internal reference. The relative correlation factor (RCF) of naringin and citrus genus glycosides B were established

and the RCF in the determination of naringin and citrus genus glycosides B was adopted. The external standard method(ESM) was used to determin the naringin and citrus genus glycosides B

and the results of the two determination methods were compared. Result: Linear experiment

hesperidin:Y=56 644X-2 272.9 (r=0.999 8)

naringin:Y=41 070X+779.59(r=0.999 9)

citrus genus glycosides B:Y=33 619X+2 289.8(r=0.999 9)

linear relationship was good;RCF

naringin:RSD 0.286%

citrus genus glycosides B:RSD 0.259%;there was no negative interference

specificity was good;precision enperiment

hesperidin:RSD 0.48%

naringin:RSD 1.02%

citrus genus glycosides B:RSD 0.97%;repeatability enperiment

hesperidin:RSD 0.94%

naringin:RSD 0.89%

citrus genus glycosides B:RSD 1.27%;recovery experiment

hesperidin:average recovery was 99.26%

RSD 0.90%

naringin:average recovery was 99.30%

RSD 0.92%

citrus genus glycosides B:average recovery was 98.34%

RSD 0.78%. The method was accurate and reliable;determination result of QAMS:naringin 6.11

6.09

6.13 mg·g-1

citrus genus glycosides B 4.96

4.93

4.99 mg·g-1;ESM:naringin 6.12

6.08

6.17 mg·g-1

citrus genus glycosides B 4.98

4.95

5.01 mg·g-1

there was no significant difference in the results of determination between the two methods. Conclusion: Using QAMS can determine the hesperidin

naringin and citrus genus glycosides B in the Shuganjianpi pills simultaneously with convenience preciseness and raliablity

which effectively improve the level of Shuganjianpi pill quality control.