TLC Identification and Content Determination of Saponins from the Roots of

LI Min; WANG Yan; YU Xian; ZHAO Lei; WANG Shu-lin

doi:10.11653/syfj2014040074

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TLC Identification and Content Determination of Saponins from the Roots of

更新时间：2024-01-04

- TLC Identification and Content Determination of Saponins from the Roots of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 4, Pages: 74-77(2014)
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- DOI：10.11653/syfj2014040074
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- Published：2014
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LI Min, WANG Yan, YU Xian, et al. TLC Identification and Content Determination of Saponins from the Roots of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 74-77.
DOI：

LI Min, WANG Yan, YU Xian, et al. TLC Identification and Content Determination of Saponins from the Roots of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 74-77. DOI： 10.11653/syfj2014040074.

摘要

目的：研究竹叶柴胡根中的皂苷类成分，为其入药提供科学依据。方法：采用薄层色谱法对皂苷类成分进行定性鉴别，并采用高效液相色谱法首次对竹叶柴胡根中皂苷类成分进行含量测定，用C18（4.60 mm×150 mm，5 μm）柱，以乙腈-水为流动相梯度洗脱，检测波长210 nm，流速0.8 mL·min-1，柱温35 ℃，进样量10 μL。结果：竹叶柴胡根中含有皂苷类成分。柴胡皂苷a和柴胡皂苷d的线性范围分别为440~8 800 ng（r=0.999 7），488~9 760 ng（r=0.999 2）线性关系良好，平均加样回收率（n=6）分别为99.88%（RSD 0.41%），100.23%（RSD 0.46%）。样品中柴胡皂苷a和柴胡皂苷d的总量在0.48%~1.08%。结论：竹叶柴胡全草入药具有其科学性。

Abstract

Objective: Studies on the saponins from the roots of Bupleurum marginatum. Method: The thin layer chromatography (TLC) was performed to identify saponins qualitatively

and a HPLC method was used for the determination of saponins in the roots of B. marginatum. The RP-HPLC system consisting of C18 (4.60 mm×150 mm

5 μm) column and a solution system of acetonitrile-water with gradient elution

the detection wavelength was at 210 nm

the flow rate was 0.8 mL·min-1

the column temperature was set at 35 ℃ and the injection volume was 10 μL. Result: The linear ranges of saikosaponin A and saikosaponin D were 440-8 800 ng (r=0.999 7)

488-9 760 ng (r=0.999 2) respectively. The average recoveries (n=6) were 99.88%(RSD 0.41%)

100.23%(RSD 0.46%) respectively. The amounts of saikosaponin A and saikosaponin D were in the range of 0.48%-1.08%. Conclusion: The whole grass of B. marginatum as a traditional chinese medicine has its scientific nature.

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