Objective: To explore the effects of ethyl acetate extract from Centella asiatica on PC12 cell Alzheirmer's disease(AD)model induced by β amyloid 25-35 fragment (Aβ25-35)and the levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the brain of SAMP8. Method: The cell suspension(1×104 cell/mL) was seeded into 96-well plates for 24 h. For preventive trial

10 μmol·L-1Aβ25-35 and drugs were added into 96-well plates. For treatment trail

drugs were added 24 h after 10 μmol·L-1 Aβ25-35 was added into the cells. After 72 h

MTT assay was used to detect the prevention and treatment effect of AD model which made in PC12 cell induced by Aβ25-35 fragment after treated with ethyl acetate extract of C. asiatica

and fluorescence microscopy was applied to observe the morphological changes of the PC12 cells. For animal trail

forty senescence accelerated mouse-prone 8(SAMP8) were divided into 5 groups. There were 8 mice in each group. Each mouse was intragastricly administrated for 2 months. The contents of SOD and GSH-Px in the brain of SAMP8 were determined by enzyme linked immunosorbent assay(ELISA). Result: Different concentrations of ethyl acetate extract had different degrees of protection on PC12 cells

the results of fluorescence microscope was the same as MTT.ELISA demonstrated that the contents of SOD in high

mid-dosage ethyl acetate extract and Huperzine(Hup-A) groups were higher than that in control group

and the contents of GSH-Px treatment with mid-dosage ethyl acetate extract and Hup-A increased compared with control group. Conclusion: Ethyl acetate extracts of C. asiaticashows some degree of protective and therapeutic effects on the damage of PC12 cell caused by the Aβ25-35 fragments in vitro. Furthermore

ethyl acetate extracts of C. asiatica has antioxidant

scavenging oxygen free radicals effects which results in delaying senescence via enhancing the contents of SOD and GSH-Px.